| RRC ID |
68097
|
| Author |
Wójtowicz K, Czogalla A, Trombik T, Łukaszewicz M.
|
| Title |
Surfactin cyclic lipopeptides change the plasma membrane composition and lateral organization in mammalian cells.
|
| Journal |
Biochim Biophys Acta Biomembr
|
| Abstract |
The specific structure and composition of the cell plasma membrane (PM) is crucial for many cellular processes and can be targeted by various substances with potential medical applications. In this context, biosurfactants (BS) constitute a promising group of natural compounds that possess several biological functions, including anticancer activity. Despite the efficiency of BS, their mode of action had never been elucidated before. Here, we demonstrate the influence of cyclic lipopeptide surfactin (SU) on the PM of CHO-K1 cells. Both FLIM and svFCS experiments show that even a low concentration of SU causes significant changes in the membrane fluidity and dynamic molecular organization. Further, we demonstrate that SU causes a relevant dose-dependent reduction of cellular cholesterol by extracting it from the PM. Finally, we show that CHO-25RA cells characterized by increased cholesterol levels are more sensitive to SU treatment than CHO-K1 cells. We propose that sterols organizing the PM raft nanodomains, constitute a potential target for SU and other biosurfactants. In our opinion, the anticancer activity of biosurfactants is directly related with the higher cholesterol content found in many cancer cells.
|
| Volume |
1863(12)
|
| Pages |
183730
|
| Published |
2021-12-1
|
| DOI |
10.1016/j.bbamem.2021.183730
|
| PII |
S0005-2736(21)00178-4
|
| PMID |
34419486
|
| MeSH |
Animals
Antineoplastic Agents / chemistry
Antineoplastic Agents / pharmacology
CHO Cells
Cell Membrane / drug effects
Cholesterol / chemistry
Cricetulus
Humans
Lipopeptides / chemistry*
Lipopeptides / pharmacology
Membrane Fluidity / drug effects
Molecular Dynamics Simulation
Peptides, Cyclic / chemistry*
Peptides, Cyclic / pharmacology
|
| IF |
3.79
|
| Resource |
| Human and Animal Cells |
CHO-K1 (SC)(RCB0403) |
