| RRC ID |
68115
|
| 著者 |
Kim J, Adachi T.
|
| タイトル |
Cell-fate decision of mesenchymal stem cells toward osteocyte differentiation is committed by spheroid culture.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Osteocytes are mechanosensory commander cells to regulate bone remodeling throughout the lifespan. While the osteocytes are known as terminally differentiated cells derived from mesenchymal stem cells, the detailed mechanisms of osteocyte differentiation remain unclear. In this study, we fabricated 3D self-organized spheroids using human mesenchymal stem cells (MSCs). Under the osteogenesis induction medium, the spheroid culture model exerted the osteocyte-likeness within 2 days compared to a conventional 2D monolayer model. Moreover, we showed that an inhibition of actin polymerization in the spheroid further up-regulated the osteocyte gene expressions. Notably, we represented that the cell condensed condition acquired in the 3D spheroid culture model determined a differentiation fate of MSCs to osteocytes. Taken together, we suggest that our self-organized spheroid model can be utilized as a new in vitro model to represent the osteocyte and to recapitulate an in vitro ossification process.
|
| 巻・号 |
11(1)
|
| ページ |
13204
|
| 公開日 |
2021-6-24
|
| DOI |
10.1038/s41598-021-92607-z
|
| PII |
10.1038/s41598-021-92607-z
|
| PMID |
34168224
|
| PMC |
PMC8225633
|
| MeSH |
Cell Culture Techniques / methods
Cell Differentiation / physiology*
Cells, Cultured
Culture Media / metabolism
Humans
Mesenchymal Stem Cells / cytology*
Mesenchymal Stem Cells / metabolism
Osteocytes / cytology*
Osteocytes / metabolism
Osteogenesis / physiology
Spheroids, Cellular / cytology*
Spheroids, Cellular / metabolism
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
MSC-R37(HMS0020)
MSC-R43(HMS0022)
MSC-R44(HMS0023)
MSC-R50(HMS0047) |
