RRC ID 68115
Author Kim J, Adachi T.
Title Cell-fate decision of mesenchymal stem cells toward osteocyte differentiation is committed by spheroid culture.
Journal Sci Rep
Abstract Osteocytes are mechanosensory commander cells to regulate bone remodeling throughout the lifespan. While the osteocytes are known as terminally differentiated cells derived from mesenchymal stem cells, the detailed mechanisms of osteocyte differentiation remain unclear. In this study, we fabricated 3D self-organized spheroids using human mesenchymal stem cells (MSCs). Under the osteogenesis induction medium, the spheroid culture model exerted the osteocyte-likeness within 2 days compared to a conventional 2D monolayer model. Moreover, we showed that an inhibition of actin polymerization in the spheroid further up-regulated the osteocyte gene expressions. Notably, we represented that the cell condensed condition acquired in the 3D spheroid culture model determined a differentiation fate of MSCs to osteocytes. Taken together, we suggest that our self-organized spheroid model can be utilized as a new in vitro model to represent the osteocyte and to recapitulate an in vitro ossification process.
Volume 11(1)
Pages 13204
Published 2021-6-24
DOI 10.1038/s41598-021-92607-z
PII 10.1038/s41598-021-92607-z
PMID 34168224
PMC PMC8225633
MeSH Cell Culture Techniques / methods Cell Differentiation / physiology* Cells, Cultured Culture Media / metabolism Humans Mesenchymal Stem Cells / cytology* Mesenchymal Stem Cells / metabolism Osteocytes / cytology* Osteocytes / metabolism Osteogenesis / physiology Spheroids, Cellular / cytology* Spheroids, Cellular / metabolism
IF 3.998
Human and Animal Cells MSC-R37(HMS0020) MSC-R43(HMS0022) MSC-R44(HMS0023) MSC-R50(HMS0047)