論文 - 詳細
| RRC ID | 68116 |
|---|---|
| 著者 | Koike H, Iwasawa K, Ouchi R, Maezawa M, Kimura M, Kodaka A, Nishii S, Thompson WL, Takebe T. |
| タイトル | Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells. |
| ジャーナル | Nat Protoc |
| Abstract |
Human organoids are emerging as a valuable resource to investigate human organ development and disease. The applicability of human organoids has been limited, partly due to the oversimplified architecture of the current technology, which generates single-tissue organoids that lack inter-organ structural connections. Thus, engineering organoid systems that incorporate connectivity between neighboring organs is a critical unmet challenge in an evolving organoid field. Here, we describe a protocol for the continuous patterning of hepatic, biliary and pancreatic (HBP) structures from a 3D culture of human pluripotent stem cells (PSCs). After differentiating PSCs into anterior and posterior gut spheroids, the two spheroids are fused together in one well. Subsequently, self-patterning of multi-organ (i.e., HBP) domains occurs within the boundary region of the two spheroids, even in the absence of any extrinsic factors. Long-term culture of HBP structures induces differentiation of the domains into segregated organs complete with developmentally relevant invagination and epithelial branching. This in-a-dish model of human hepato-biliary-pancreatic organogenesis provides a unique platform for studying human development, congenital disorders, drug development and therapeutic transplantation. More broadly, our approach could potentially be used to establish inter-organ connectivity models for other organ systems derived from stem cell cultures. |
| 巻・号 | 16(2) |
| ページ | 919-936 |
| 公開日 | 2021-2-1 |
| DOI | 10.1038/s41596-020-00441-w |
| PII | 10.1038/s41596-020-00441-w |
| PMID | 33432231 |
| PMC | PMC8212777 |
| MeSH | Bile Ducts / cytology Cell Culture Techniques / methods* Cell Differentiation / physiology Humans Liver / cytology Organogenesis / physiology Organoids / cytology* Organoids / metabolism Pancreas / cytology Pluripotent Stem Cells / cytology Pluripotent Stem Cells / metabolism Tissue Engineering / methods* |
| IF | 10.419 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 40 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 6.0 |
| リソース情報 | |
| ヒト・動物細胞 | 409B2(HPS0076) |