論文 - 詳細
| RRC ID | 68122 |
|---|---|
| 著者 | Li Y, Liu YD, Zhou XY, Zhang J, Wu XM, Yang YZ, Chen YX, Zhang XF, Li X, Ma LZ, Wang Z, Chen SL. |
| タイトル | Let-7e modulates the proliferation and the autophagy of human granulosa cells by suppressing p21 signaling pathway in polycystic ovary syndrome without hyperandrogenism. |
| ジャーナル | Mol Cell Endocrinol |
| Abstract |
Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in reproductive-aged women, and its pathogenesis is still under debate. Recent studies suggest crucial roles for microRNAs (miRNAs) in PCOS development. The let-7 family miRNAs constitute the most abundant miRNAs in human granulosa cells (GCs), and plays an important role in follicular development. However, research on the let-7e implications of the non-hyperandrogenic (non-HA) phenotype remains unclear. This study aimed at determining the role of let-7e in the progression of PCOS. We performed quantitative real-time PCR to examine the levels of let-7e in fifty-two non-HA PCOS patients and fifty-two controls. A receiver operating characteristic (ROC) curve were used to reveal the diagnostic value of let-7e in non-HA PCOS. Using an immortalized human granulosa cell line, KGN, we investigated the influence of let-7e on cell proliferation and autophagy. Our data substantiated the expression of let-7e was significantly increased in non-HA PCOS group, and associated with an increased antral follicle count. The ROC curve indicated a major separation between non-HA PCOS group and the control group. Let-7e knockdown suppressed cell proliferation and enhanced cell autophagy by activating p21 pathway. Conversely, let-7e overexpression promoted cell proliferation and inhibited cell autophagy by suppressing p21 pathway. Our results indicate that increased let-7e levels in non-HA PCOS GCs may contribute to excessive follicular activation and growth, thereby involving in the pathogenesis of PCOS. Let-7e may thus be a potential therapeutic target in non-HA PCOS. |
| 巻・号 | 535 |
| ページ | 111392 |
| 公開日 | 2021-9-15 |
| DOI | 10.1016/j.mce.2021.111392 |
| PII | S0303-7207(21)00236-7 |
| PMID | 34246727 |
| MeSH | Adult Autophagy Case-Control Studies Cell Line Cell Proliferation Cyclin-Dependent Kinase Inhibitor p21 / genetics* Female Granulosa Cells / cytology* Granulosa Cells / metabolism Humans Hyperandrogenism / genetics* MicroRNAs / genetics* Polycystic Ovary Syndrome / genetics* Signal Transduction Up-Regulation |
| IF | 3.871 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 5.0 |
| リソース情報 | |
| ヒト・動物細胞 | KGN(RCB1154) |