Reference - Detail
| RRC ID | 68133 |
|---|---|
| Author | Horikawa A, Mizuno K, Tsuda K, Yamamoto T, Michiue T. |
| Title | A simple method of hiPSCs differentiation into insulin-producing cells is improved with vitamin C and RepSox. |
| Journal | PLoS One |
| Abstract |
Human induced pluripotent stem cells (hiPSCs) are considered a promising source of pancreatic β-cells for the treatment of diabetes. However, this approach is limited by issues such as low efficiency and high cost. Here, we have developed a new protocol to induce insulin-producing cells. To reduce costs, we decreased the number of reagents and replaced protein reagents with chemical compounds. In this method, we increased induction efficiency with ascorbic acid (vitamin C) and an ALK5 inhibitor, RepSox. In 2D culture, the majority of cells were immature β-cells with low glucose-stimulated insulin secretion. Transferring to 3D culture immediately after endocrine progenitor cell differentiation, however, improved glucose-stimulated insulin secretion. This simplified method will contribute to realizing transplantation therapy of β-cells using iPSCs. |
| Volume | 16(7) |
| Pages | e0254373 |
| Published | 2021-1-1 |
| DOI | 10.1371/journal.pone.0254373 |
| PII | PONE-D-21-09954 |
| PMID | 34252142 |
| PMC | PMC8274930 |
| MeSH | Animals Ascorbic Acid / pharmacology* Cell Count Cell Culture Techniques / methods* Cell Differentiation* Endoderm / cytology Glucose / pharmacology Humans Induced Pluripotent Stem Cells / cytology* Insulin Secretion / drug effects Insulin-Secreting Cells / cytology* Mice Pyrazoles / pharmacology* Pyridines / pharmacology* |
| IF | 2.74 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 201B7(HPS0063) |