論文 - 詳細
| RRC ID | 6820 |
|---|---|
| 著者 | Shinozaki A, Sakatani T, Ushiku T, Hino R, Isogai M, Ishikawa S, Uozaki H, Takada K, Fukayama M. |
| タイトル | Downregulation of microRNA-200 in EBV-associated gastric carcinoma. |
| ジャーナル | Cancer Res |
| Abstract |
EBV-associated gastric carcinoma is a distinct gastric carcinoma subtype with characteristic morphologic features similar to those of cells that undergo epithelial-to-mesenchymal transition. The effect of microRNA abnormalities in carcinogenesis was investigated by measuring the expression of the epithelial-to-mesenchymal transition-related microRNAs, miR-200a and miR-200b, in 36 surgically resected gastric carcinomas using quantitative reverse transcription-PCR analysis. MiR-200 family expression was decreased in EBV-associated gastric carcinoma, as compared with that in EBV-negative carcinoma. Downregulation of the miR-200 family was found in gastric carcinoma cell lines infected with recombinant EBV (MKN74-EBV, MKN7-EBV, and NUGC3-EBV), accompanied by the loss of cell adhesion, reduction of E-cadherin expression, and upregulation of ZEB1 and ZEB2. E-cadherin expression was partially restored by transfection of EBV-infected cells with miR-200 family precursors. Reverse transcription-PCR analysis of primary precursors of miR-200 (pri-miR-200) revealed that the transcription of pri-miR-200 was decreased in EBV-infected cells. Transfection of MKN74 cells with BARF0, EBNA1, and LMP2A resulted in a decrease of pri-miR-200, whereas transfection with EBV-encoded small RNA (EBER) did not. These four latent genes contributed to the downregulation of the mature miR-200 family and the subsequent upregulation of ZEB1/ZEB2, resulting in the reduction of E-cadherin expression. These findings indicate that all the latency type I genes have a synergetic effect on the downregulation of the miR-200 family that leads to reduced E-cadherin expression, which is a crucial step in the carcinogenesis of EBV-associated gastric carcinoma. |
| 巻・号 | 70(11) |
| ページ | 4719-27 |
| 公開日 | 2010-6-1 |
| DOI | 10.1158/0008-5472.CAN-09-4620 |
| PII | 0008-5472.CAN-09-4620 |
| PMID | 20484038 |
| MeSH | Cadherins / biosynthesis Cadherins / genetics Cell Line, Tumor Down-Regulation Epstein-Barr Virus Infections / genetics* Epstein-Barr Virus Infections / metabolism Epstein-Barr Virus Infections / pathology* Gene Expression Regulation, Neoplastic Gene Expression Regulation, Viral Herpesvirus 4, Human / genetics Herpesvirus 4, Human / metabolism Herpesvirus 4, Human / physiology* Humans MicroRNAs / biosynthesis MicroRNAs / genetics* Reverse Transcriptase Polymerase Chain Reaction Stomach Neoplasms / genetics* Stomach Neoplasms / metabolism Stomach Neoplasms / pathology Stomach Neoplasms / virology* Transfection Virus Latency |
| IF | 9.727 |
| 引用数 | 95 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MKN7(RCB0999) MKN74(RCB1002) |