RRC ID 6835
Author Masson P, Lundin D, Söderbom F, Young P.
Title Characterization of a REG/PA28 proteasome activator homolog in Dictyostelium discoideum indicates that the ubiquitin- and ATP-independent REGgamma proteasome is an ancient nuclear protease.
Journal Eukaryot Cell
Abstract The nuclear proteasome activator REGgamma/PA28gamma is an ATP- and ubiquitin-independent activator of the 20S proteasome and has been proposed to degrade and thereby regulate both a key human oncogene, encoding the coactivator SRC-3/AIB1, and the cyclin-dependent kinase inhibitor p21 (Waf/Cip1). We report the identification and characterization of a PA28/REG homolog in Dictyostelium. Association of a recombinant Dictyostelium REG with the purified Dictyostelium 20S proteasome led to the preferential stimulation of the trypsin-like proteasome peptidase activity. Immunolocalization studies demonstrated that the proteasome activator is localized to the nucleus and is present in growing as well as starving Dictyostelium cells. Our results indicate that the Dictyostelium PA28/REG activator can stimulate both the trypsin-like and chymotrypsin-like activities of the 20S proteasome and supports the idea that the REGgamma-20S proteasome represents an early unique nuclear degradation pathway for eukaryotic cells.
Volume 8(6)
Pages 844-51
Published 2009-6-1
DOI 10.1128/EC.00165-08
PII EC.00165-08
PMID 19411624
PMC PMC2698304
MeSH Adenosine Triphosphate / metabolism* Amino Acid Sequence Animals Dictyostelium / chemistry Dictyostelium / genetics Dictyostelium / metabolism* Molecular Sequence Data Nuclear Proteins / chemistry Nuclear Proteins / genetics Nuclear Proteins / metabolism* Peptide Hydrolases / chemistry Peptide Hydrolases / genetics Peptide Hydrolases / metabolism* Proteasome Endopeptidase Complex / chemistry Proteasome Endopeptidase Complex / genetics Proteasome Endopeptidase Complex / metabolism* Protein Transport Protozoan Proteins / chemistry Protozoan Proteins / genetics Protozoan Proteins / metabolism* Sequence Alignment Ubiquitin / metabolism*
IF 2.992
Times Cited 11
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