論文 - 詳細
| RRC ID | 68409 |
|---|---|
| 著者 | Maeshima K, Yahata K, Sasaki Y, Nakatomi R, Tachibana T, Hashikawa T, Imamoto F, Imamoto N. |
| タイトル | Cell-cycle-dependent dynamics of nuclear pores: pore-free islands and lamins. |
| ジャーナル | J Cell Sci |
| Abstract |
Nuclear pores are sophisticated gateways on the nuclear envelope that control macromolecular transport between the cytoplasm and nucleoplasm. So far the structural and functional aspects of nuclear pores have been extensively studied, but their distribution and density, which might reflect nuclear organization and function, remain unknown. Here, we report the cell-cycle-dependent dynamics of nuclear pores. Large distinct subdomains lacking nuclear pores are present on the nuclear surface of HeLaS3 cells in early cell-cycle stages. Such ;pore-free islands' gradually become dispersed in G1-S phase. Surprisingly, the islands are enriched with inner nuclear membrane proteins lamin A/C and emerin, but exclude lamin B. Lamin-A/C-enriched pore-free islands were also observed in human normal diploid fibroblasts and several cell lines, showing the generality of this phenomenon. Knockdown and ectopic expression analyses demonstrated that lamin A/C, but not emerin, plays an essential structural and regulatory role in the nuclear pore distribution and the formation of pore-free islands. These data thus provide strong evidence that the dynamics of nuclear pores are regulated by the reorganization of inner nuclear structures. |
| 巻・号 | 119(Pt 21) |
| ページ | 4442-51 |
| 公開日 | 2006-11-1 |
| DOI | 10.1242/jcs.03207 |
| PII | 119/21/4442 |
| PMID | 17074834 |
| MeSH | Bone Neoplasms / metabolism Cell Cycle / physiology* Cell Nucleus / ultrastructure Cells, Cultured Fibroblasts / cytology Fibroblasts / metabolism Fluorescent Antibody Technique HeLa Cells / metabolism Humans Lamin Type A / genetics Lamin Type A / metabolism* Lamin Type B / genetics Lamin Type B / metabolism Mitosis / physiology Nuclear Envelope Nuclear Pore / physiology* Osteosarcoma / metabolism Plasmids |
| IF | 4.573 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa.S3(RCB0191) HeLa(RCB0007) |