Reference - Detail
| RRC ID | 68431 |
|---|---|
| Author | Sasaki M, Kishimoto M, Itakura Y, Tabata K, Intaruck K, Uemura K, Toba S, Sanaki T, Sato A, Hall WW, Orba Y, Sawa H. |
| Title | Air-liquid interphase culture confers SARS-CoV-2 susceptibility to A549 alveolar epithelial cells. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
The human lung cell A549 is susceptible to infection with a number of respiratory viruses. However, A549 cells are resistant to Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) infection in conventional submerged culture, and this would appear to be due to low expression levels of the SARS-CoV-2 entry receptor: angiotensin-converting enzyme-2 (ACE2). Here, we examined SARS-CoV-2 susceptibility to A549 cells after adaptation to air-liquid interface (ALI) culture. A549 cells in ALI culture yielded a layer of mucus on their apical surface, exhibited decreased expression levels of the proliferation marker KI-67 and intriguingly became susceptible to SARS-CoV-2 infection. We found that A549 cells increased the endogenous expression levels of ACE2 and TMPRSS2 following adaptation to ALI culture conditions. Camostat, a TMPRSS2 inhibitor, reduced SARS-CoV-2 infection in ALI-cultured A549 cells. These findings indicate that ALI culture switches the phenotype of A549 cells from resistance to susceptibility to SARS-CoV-2 infection through upregulation of ACE2 and TMPRSS2. |
| Volume | 577 |
| Pages | 146-151 |
| Published | 2021-11-5 |
| DOI | 10.1016/j.bbrc.2021.09.015 |
| PII | S0006-291X(21)01309-7 |
| PMID | 34517212 |
| PMC | PMC8423671 |
| MeSH | A549 Cells Alveolar Epithelial Cells / pathology Alveolar Epithelial Cells / virology* COVID-19 / virology* Cell Culture Techniques / methods* Cells, Cultured Disease Susceptibility Gene Expression Regulation, Neoplastic Humans Peptidyl-Dipeptidase A / genetics Peptidyl-Dipeptidase A / metabolism SARS-CoV-2 / physiology* Serine Endopeptidases / genetics Serine Endopeptidases / metabolism Up-Regulation / genetics |
| IF | 2.985 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | A549(RCB0098) |