Reference - Detail
| RRC ID | 68450 |
|---|---|
| Author | Koshimura K, Murakami Y, Sohmiya M, Tanaka J, Kato Y. |
| Title | Effects of erythropoietin on neuronal activity. |
| Journal | J Neurochem |
| Abstract |
Recently, erythropoietin (EPO) receptors and synthesis of EPO have been identified in the brain. To clarify the effects of EPO on neuronal cells, we investigated the effects of EPO on Ca2+ uptake, intracellular Ca2+ concentration, membrane potential, cell survival, release and biosynthesis of dopamine, and nitric oxide (NO) production in differentiated PC12 cells, which possess EPO receptors. EPO (10(-12)-10(-10) M) increased 45Ca2+ uptake and intracellular Ca2+ concentration in PC12 cells in a dose-related manner; these increases were inhibited by nicardipine (1 microM) or anti-EPO antibody (1:100 dilution). EPO induced membrane depolarization in PC12 cells. After a 5-day culture without serum and nerve growth factor (NGF), viable cell number decreased to 50% of that of the control cells cultured with serum and NGF. EPO (10(-13)-10(-10) M) increased the number of viable cells cultured without serum and NGF; this increase was blunted by nicardipine or anti-EPO antibody. Incubation with EPO (10(-13)-10(-10) M) stimulated mitogen-activated protein kinase activity in PC12 cells. EPO (10(-13)-10(-10) M) increased dopamine release from PC12 cells and tyrosine hydroxylase activity; these increases were sensitive to nicardipine or anti-EPO antibody. Following a 4-h incubation with EPO (10(-14)-10(-10) M), NO production was increased, which was blunted by nicardipine and anti-EPO antibody. In contrast, maximal NO synthase activity was not changed by EPO. These results suggest that EPO stimulates neuronal function and viability via activation of Ca2+ channels. |
| Volume | 72(6) |
| Pages | 2565-72 |
| Published | 1999-6-1 |
| DOI | 10.1046/j.1471-4159.1999.0722565.x |
| PMID | 10349868 |
| MeSH | Animals Antibodies / pharmacology Biological Transport Calcium / metabolism* Calcium-Calmodulin-Dependent Protein Kinases / metabolism Cell Differentiation / drug effects Cell Membrane / drug effects Cell Membrane / physiology* Cell Survival / drug effects Dopamine / metabolism Erythropoietin / immunology Erythropoietin / pharmacology* Humans Membrane Potentials / drug effects Membrane Potentials / physiology Nerve Growth Factors / pharmacology Neurons / cytology Neurons / drug effects Neurons / physiology* Nicardipine / pharmacology Nitric Oxide / metabolism PC12 Cells Rats Recombinant Proteins Tyrosine 3-Monooxygenase / metabolism |
| IF | 4.066 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 11 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | PC-12(RCB0009) |