論文 - 詳細
| RRC ID | 6847 |
|---|---|
| 著者 | Kimura Y, Kurabe N, Ikegami K, Tsutsumi K, Konishi Y, Kaplan OI, Kunitomo H, Iino Y, Blacque OE, Setou M. |
| タイトル | Identification of tubulin deglutamylase among Caenorhabditis elegans and mammalian cytosolic carboxypeptidases (CCPs). |
| ジャーナル | J Biol Chem |
| Abstract |
Tubulin polyglutamylation is a reversible post-translational modification, serving important roles in microtubule (MT)-related processes. Polyglutamylases of the tubulin tyrosine ligase-like (TTLL) family add glutamate moieties to specific tubulin glutamate residues, whereas as yet unknown deglutamylases shorten polyglutamate chains. First we investigated regulatory machinery of tubulin glutamylation in MT-based sensory cilia of the roundworm Caenorhabditis elegans. We found that ciliary MTs were polyglutamylated by a process requiring ttll-4. Conversely, loss of ccpp-6 gene function, which encodes one of two cytosolic carboxypeptidases (CCPs), resulted in elevated levels of ciliary MT polyglutamylation. Consistent with a deglutamylase function for ccpp-6, overexpression of this gene in ciliated cells decreased polyglutamylation signals. Similarly, we confirmed that overexpression of murine CCP5, one of two sequence orthologs of nematode ccpp-6, caused a dramatic loss of MT polyglutamylation in cultured mammalian cells. Finally, using an in vitro assay for tubulin glutamylation, we found that recombinantly expressed Myc-tagged CCP5 exhibited deglutamylase biochemical activities. Together, these data from two evolutionarily divergent systems identify C. elegans CCPP-6 and its mammalian ortholog CCP5 as a tubulin deglutamylase. |
| 巻・号 | 285(30) |
| ページ | 22936-41 |
| 公開日 | 2010-7-23 |
| DOI | 10.1074/jbc.C110.128280 |
| PII | S0021-9258(20)60335-0 |
| PMID | 20519502 |
| PMC | PMC2906286 |
| MeSH | Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Carboxypeptidases / chemistry* Carboxypeptidases / genetics Carboxypeptidases / metabolism* Cell Line Cilia / metabolism Cytosol / enzymology* Humans Mice Microtubules / metabolism Peptide Synthases / chemistry* Peptide Synthases / genetics Peptide Synthases / metabolism* Protein Processing, Post-Translational Sequence Homology, Amino Acid |
| IF | 4.238 |
| 引用数 | 60 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3360 tm3889 tm3871 tm3310 |