RRC ID 68485
著者 Enosawa S, Suzuki S, Fujino M, Amemiya H, Omasa T, Urayama S, Tanimura N, Suga K.
タイトル An attempt to add biological functions by genetic engineering in order to produce high-performance bioreactor cells for hybrid artificial liver: transfection of glutamine synthetase into Chinese hamster ovary (CHO) cell.
ジャーナル Cell Transplant
Abstract In the course of immortalization, hepatocyte cell lines lose their original differentiated functions, such as ammonia removal and urea formation, drug metabolism, serum protein synthesis, etc. (Enosawa et al., Cell Transplant. 5:S39-S40; 1996). With the aim of adding lost or deficient functions and producing cell lines for the bioreactor of a hybrid artificial liver, rat glutamine synthetase (GS) gene was transfected into Chinese hamster ovary (CHO) cells, because it is able to lower the ammonia level. The GS gene-inserted pSV2 plasmid was transfected into the CHO-K1 line by electroporation. Transfected CHO (GS-CHO) cells were cultured in a glutamine-free medium containing ammonia, glutamic acid, and the GS inhibitor methionine sulfoximine (MSX). The MSX concentration was increased stepwise from 25 mumol/L to 1600 mumol/L to amplify the GS gene. In several GS-CHO sublines resistant to 300-1600 mumol/L of MSX, the specific activities of GS were increased from 0.2 x 10(4) to 1.7-2.9 x 10(4) unit/10(6) cells. When the amplified GS-CHO cells were cultured in the ammonia-containing medium, a slow but steady decrease of the ammonia level was observed when the level was high. Finally, the prospect of genetically modulated cells for bioreactors in the hybrid artificial liver is discussed.
巻・号 6(5)
ページ 537-40
公開日 1997-1-1
DOI 10.1177/096368979700600518
PII S0963689797000730
PMID 9331509
MeSH Ammonia / metabolism Animals Bioreactors CHO Cells* Cricetinae Enzyme Inhibitors / pharmacology Gene Expression Regulation, Enzymologic / drug effects Genetic Engineering / methods* Glutamate-Ammonia Ligase / antagonists & inhibitors Glutamate-Ammonia Ligase / genetics* Liver, Artificial* Methionine Sulfoximine / pharmacology Rats Transfection
IF 3.341
リソース情報
ヒト・動物細胞 CHO-K1(RCB0285)