Reference - Detail
| RRC ID | 68489 |
|---|---|
| Author | Anuradha CD, Kanno S, Hirano S. |
| Title | RGD peptide-induced apoptosis in human leukemia HL-60 cells requires caspase-3 activation. |
| Journal | Cell Biol Toxicol |
| Abstract |
RGD motif-containing peptides have been used in various studies of cell adhesion and growth. We report that RGD triggered apoptosis at a concentration of 1 mmol/L, whereas RAD-containing peptides failed to induce apoptosis in HL-60 cells. RGD-treated cells revealed internucleosomal DNA fragmentation. Western blot reveals caspase-3 activation in RGD peptide-treated cells. A caspase-3 inhibitor z-VAD-FMK completely blocked the apoptosis, but a caspase-1 inhibitor (Ac-YVAD-CMK) and caspase-2 inhibitor (z-VDVAD-FMK) did not block the apoptosis, suggesting that caspase-3 might have a critical role in the execution process of apoptosis induced by RGD. RGD peptides have been used extensively to inhibit tumor metastasis. Our results should help in further understanding the RGD peptide-induced apoptosis, which is important since RGD peptides have a potential role in therapies of the future. |
| Volume | 16(5) |
| Pages | 275-83 |
| Published | 2000-1-1 |
| DOI | 10.1023/a:1026758429238 |
| PMID | 11201051 |
| MeSH | Amino Acid Chloromethyl Ketones / pharmacology Annexins / metabolism Apoptosis / drug effects* Caspase 3 Caspase Inhibitors Caspases / metabolism* Cysteine Proteinase Inhibitors / pharmacology Enzyme Activation HL-60 Cells Humans Oligopeptides / pharmacology* |
| IF | 6.284 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HL60(RCB0041) |