| RRC ID |
68506
|
| Author |
Murata-Hori M, Fukuta Y, Ueda K, Iwasaki T, Hosoya H.
|
| Title |
HeLa ZIP kinase induces diphosphorylation of myosin II regulatory light chain and reorganization of actin filaments in nonmuscle cells.
|
| Journal |
Oncogene
|
| Abstract |
Dlk/ZIP kinase is a serine/threonine kinase highly homologous to DAP kinase. We have reported that HeLa ZIP kinase (hZIPK) phosphorylated the regulatory light chain of myosin II (MRLC) at both Ser19 and Thr18 in vitro. In this study, we demonstrate that hZIPK also induces the diphosphorylation of MRLC in nonmuscle cells. Peptide mapping revealed that transient transfection of hZIPK into HeLa cells caused diphosphorylation of MRLC. In contrast, transfection of the kinase inactive mutant of hZIPK did not induce any phosphorylation of MRLC. Using antibodies specific for mono- or diphosphorylated MRLC, we showed that diphosphorylated MRLC induced by the overexpression of hZIPK was concentrated in striking aggregates or bundles of actin filaments in HeLa cells, while monophosphorylated MRLC showed no prominent localization to these aggregates. Overexpression of hZIPK also induced dramatic changes in cell shape and disruption of nuclear morphology reminiscent of changes during apoptosis. These effects of hZIPK were suppressed by the coexpression of a mutant MRLC where both phosphorylation sites were replaced with alanine, indicating that the changes in actin organization were a consequence of MRLC diphosphorylation. These results suggested that hZIPK plays a role in regulating actin organization and cell morphology in non-muscles and at least part of its effects are mediated through the diphosphorylation of MRLC.
|
| Volume |
20(57)
|
| Pages |
8175-83
|
| Published |
2001-12-13
|
| DOI |
10.1038/sj.onc.1205055
|
| PMID |
11781833
|
| MeSH |
Actin Cytoskeleton / ultrastructure*
Actins / metabolism*
Antibodies / immunology
Antibody Specificity
Apoptosis Regulatory Proteins
Calcium-Calmodulin-Dependent Protein Kinases
Cell Nucleus / ultrastructure
Death-Associated Protein Kinases
HeLa Cells
Humans
Microscopy, Fluorescence
Muscles / metabolism
Mutation
Myosin Light Chains / immunology
Myosin Light Chains / metabolism*
Myosin Type II / metabolism*
Peptide Mapping
Phosphorylation
Protein Serine-Threonine Kinases / genetics
Protein Serine-Threonine Kinases / physiology*
Transfection
|
| IF |
7.971
|
| Resource |
| Human and Animal Cells |
HeLa(RCB0007)
REF-RGB(RCB0165) |
