RRC ID 68571
Author Tabata K, Sugano E, Hatakeyama A, Watanabe Y, Suzuki T, Ozaki T, Fukuda T, Tomita H.
Title Phototoxicities Caused by Continuous Light Exposure Were Not Induced in Retinal Ganglion Cells Transduced by an Optogenetic Gene.
Journal Int J Mol Sci
Abstract The death of photoreceptor cells is induced by continuous light exposure. However, it is unclear whether light damage was induced in retinal ganglion cells with photosensitivity by transduction of optogenetic genes. In this study, we evaluated the phototoxicities of continuous light exposure on retinal ganglion cells after transduction of the optogenetic gene mVChR1 using an adeno-associated virus vector. Rats were exposed to continuous light for a week, and visually evoked potentials (VEPs) were recorded. The intensities of continuous light (500, 1000, 3000, and 5000 lx) increased substantially after VEP recordings. After the final recording of VEPs, retinal ganglion cells (RGCs) were retrogradely labeled with a fluorescein tracer, FluoroGold, and the number of retinal ganglion cells was counted under a fluorescent microscope. There was no significant reduction in the amplitudes of VEPs and the number of RGCs after exposure to any light intensity. These results indicated that RGCs were photosensitive after the transduction of optogenetic genes and did not induce any phototoxicity by continuous light exposure.
Volume 22(13)
Published 2021-6-23
DOI 10.3390/ijms22136732
PII ijms22136732
PMID 34201658
PMC PMC8269149
MeSH Animals Dependovirus / genetics Evoked Potentials, Visual Fluorescent Dyes / chemistry Fluorescent Dyes / metabolism HEK293 Cells Humans Light / adverse effects Optogenetics / methods* Patch-Clamp Techniques Photic Stimulation Rats Retinal Ganglion Cells / pathology Retinal Ganglion Cells / physiology* Rhodopsin / genetics* Rhodopsin / metabolism Stilbamidines / chemistry Stilbamidines / metabolism Transduction, Genetic Volvox / genetics
IF 4.556
Resource
Human and Animal Cells 293(RCB1637)