| RRC ID |
68572
|
| 著者 |
Nakamura T, Fukiage M, Higuchi M, Nakaya A, Yano I, Miyazaki J, Nishiyama H, Akaza H, Ito T, Hosokawa H, Nakayama T, Harashima H.
|
| タイトル |
Nanoparticulation of BCG-CWS for application to bladder cancer therapy.
|
| ジャーナル |
J Control Release
|
| Abstract |
The Mycobacterium bovis Bacille Calmette-Guerin cell wall skeleton (BCG-CWS) could be used to replace live BCG as a bladder cancer drug. However, because BCG-CWS is poorly soluble, has a strong-negative charge, very high molecular weight and heterogeneity in size of tens of μm, it cannot be used in such an application. We report herein on the development of a novel packaging method that permits BCG-CWS to be encapsulated into 166nm-sized lipid particles. The BCG-CWS encapsulated nano particle (CWS-NP) has a high uniformity and can be easily dispersed. Thus, it has the potential for use as a packaging method that would advance the scope of applications of BCG-CWS as a bladder cancer drug. In a functional evaluation, CWS-NP was efficiently taken up by mouse bladder tumor (MBT-2) cells in vitro and inhibited tumor growth in mice bearing MBT-2 tumors. Moreover, intravesically administered CWS-NP showed significant antitumor effects in a rat model with naturally developed bladder cancer. An enhancement in Th1 differentiation by CWS-NP was also confirmed in human T cells. In conclusion, CWS-NP represents a promising delivery system for BCG-CWS for clinical development as a potent bladder cancer drug.
|
| 巻・号 |
176
|
| ページ |
44-53
|
| 公開日 |
2014-2-28
|
| DOI |
10.1016/j.jconrel.2013.12.027
|
| PII |
S0168-3659(13)00969-3
|
| PMID |
24389133
|
| MeSH |
Adult
Animals
Antineoplastic Agents / administration & dosage*
Butylhydroxybutylnitrosamine
Cell Differentiation / drug effects
Cell Line, Tumor
Cell Wall Skeleton / administration & dosage*
Cells, Cultured
Female
Humans
Male
Mice
Mice, Inbred C3H
Mycobacterium bovis*
Nanoparticles / administration & dosage*
Rats
Rats, Inbred F344
T-Lymphocytes / cytology
T-Lymphocytes / drug effects
Urinary Bladder Neoplasms / chemically induced
Urinary Bladder Neoplasms / drug therapy*
Young Adult
|
| IF |
7.727
|
| リソース情報 |
| ヒト・動物細胞 |
MBT-2(RCB0544) |
