論文 - 詳細
| RRC ID | 68592 |
|---|---|
| 著者 | Ushida Y, Shimokawa Y, Toida T, Matsui H, Takase M. |
| タイトル | Bovine alpha-lactalbumin stimulates mucus metabolism in gastric mucosa. |
| ジャーナル | J Dairy Sci |
| Abstract |
Bovine alpha-lactalbumin (alpha-LA), a major milk protein, exerts strong gastroprotective activity against rat experimental gastric ulcers induced by ethanol or stress. To elucidate the mechanisms underlying this activity, the influence of alpha-LA on gastric mucus metabolism was investigated in vitro and in vivo. For the in vitro study, RGM1 cells (a rat gastric epithelial cell line) were selected for observation of the direct activity of alpha-LA on gastric mucosal cells and cultured in the presence of either alpha-LA or ovalbumin (OVA), a reference protein showing no gastroprotective activity. Amounts of synthesized and secreted mucin, a major component of mucus, were determined using [3H]glucosamine as a tracer, and prostaglandin E2 (PGE2) levels in the culture medium were determined by RIA. For the in vivo study, the thickness of the mucus gel layer, a protective barrier for gastric mucosa, was evaluated histochemically in rat gastric mucosa. alpha-Lactalbumin (3 mg/mL) significantly stimulated mucin synthesis and secretion in RGM1 cells and also increased PGE2 levels in the culture medium. In contrast, OVA showed no enhancing effects under identical conditions. Neither indomethacin, a cyclo-oxygenase inhibitor, nor AH23848, a prostaglandin EP4 receptor antagonist, affected alpha-LA-induced enhancement of mucin synthesis and secretion. In vivo, oral administration of alpha-LA (300 mg/kg x 3 times/d x 7 d) increased the thickness of the mucus gel layer in rats. These results indicate that alpha-LA fortifies the mucus gel layer by stimulating mucin production and secretion in gastric mucus-producing cells, and that this enhancing effect is independent of endogenous PGE2. Comparison of the efficacy of alpha-LA with OVA suggests that the activities observed in RGM1 cells are closely related to the gastroprotective effects in rat gastric ulcer models. In conclusion, alpha-LA stimulates mucus metabolism, and this action may be responsible for its gastroprotective activity. |
| 巻・号 | 90(2) |
| ページ | 541-6 |
| 公開日 | 2007-2-1 |
| DOI | 10.3168/jds.S0022-0302(07)71537-0 |
| PII | S0022-0302(07)71537-0 |
| PMID | 17235130 |
| MeSH | Animals Cattle Cell Line Culture Media, Conditioned / analysis Dinoprostone / analysis Gastric Mucosa / anatomy & histology Gastric Mucosa / drug effects* Gastric Mucosa / metabolism* Glucosamine / metabolism Histocytochemistry In Vitro Techniques Lactalbumin / administration & dosage Lactalbumin / pharmacology* Male Mucins / biosynthesis Mucins / metabolism Mucus / metabolism* Ovalbumin / pharmacology Rats Rats, Wistar Stomach Ulcer / prevention & control Tritium |
| IF | 3.333 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RGM1(RCB0876) |