論文 - 詳細
| RRC ID | 68607 |
|---|---|
| 著者 | Hirose N, Hua Z, Kato Y, Zhang Q, Li R, Nishimura K, Masuda M. |
| タイトル | Molecular Characterization of Prototheca strains isolated in China revealed the first cases of protothecosis associated with Prototheca zopfii genotype 1. |
| ジャーナル | Med Mycol |
| Abstract |
In this study, six strains of Prototheca isolated in China from human patients diagnosed as protothecosis and cows with mastitis were characterized by polymerase chain reaction (PCR) and nucleotide sequencing of the ribosomal RNA gene (rDNA) and by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). The results indicated that three strains isolated from the human patients were P. zopfii genotype 1, revealing the first cases of human protothecosis associated with P. zopfii genotype 1. The remaining three strains were shown to be P. zopfii genotype 2. Interestingly, one strain isolated from the cerebrospinal fluid of the human patient appeared to have both of the genotype 1- and 2-specific alleles in the small subunit (SSU) rDNA although it was classified by MALDI-TOF MS as genotype 2. For genotyping of certain strains of P. zopfii, it may be necessary to comprehensively evaluate the diversity in the SSU rDNA sequences and the MALDI-TOF MS results. |
| 巻・号 | 56(3) |
| ページ | 279-287 |
| 公開日 | 2018-4-1 |
| DOI | 10.1093/mmy/myx039 |
| PII | 3835469 |
| PMID | 28525645 |
| MeSH | Animals Base Sequence Cattle DNA, Ribosomal / genetics Female Genotype Humans Infections / parasitology Infections / pathology* Mastitis, Bovine / parasitology* Phylogeny Prototheca / classification Prototheca / genetics* Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| IF | 2.822 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原真核微生物 | Prototheca miyajii IFM 53848 |