論文 - 詳細
| RRC ID | 68617 |
|---|---|
| 著者 | Kuribayashi-Shigetomi K, Onoe H, Takeuchi S. |
| タイトル | Cell origami: self-folding of three-dimensional cell-laden microstructures driven by cell traction force. |
| ジャーナル | PLoS One |
| Abstract |
This paper describes a method of generating three-dimensional (3D) cell-laden microstructures by applying the principle of origami folding technique and cell traction force (CTF). We harness the CTF as a biological driving force to fold the microstructures. Cells stretch and adhere across multiple microplates. Upon detaching the microplates from a substrate, CTF causes the plates to lift and fold according to a prescribed pattern. This self-folding technique using cells is highly biocompatible and does not involve special material requirements for the microplates and hinges to induce folding. We successfully produced various 3D cell-laden microstructures by just changing the geometry of the patterned 2D plates. We also achieved mass-production of the 3D cell-laden microstructures without causing damage to the cells. We believe that our methods will be useful for biotechnology applications that require analysis of cells in 3D configurations and for self-assembly of cell-based micro-medical devices. |
| 巻・号 | 7(12) |
| ページ | e51085 |
| 公開日 | 2012-1-1 |
| DOI | 10.1371/journal.pone.0051085 |
| PII | PONE-D-12-15395 |
| PMID | 23251426 |
| PMC | PMC3521028 |
| MeSH | Animals Cell Shape* Mice NIH 3T3 Cells |
| IF | 2.74 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 229 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NIH/3T3(RCB2767) |