RRC ID 6863
Author Wang S, Kawashima N, Sakamoto K, Katsube K, Umezawa A, Suda H.
Title Osteogenic differentiation of mouse mesenchymal progenitor cell, Kusa-A1 is promoted by mammalian transcriptional repressor Rbpj.
Journal Biochem Biophys Res Commun
Abstract Pluripotent mesenchymal stem cells possess the ability to differentiate into many cell types, but the precise mechanisms of differentiation are still unclear. Here, we provide evidence that Rbpj (recombination signal-binding protein for immunoglobulin kappa j region) protein, the primary nuclear mediator of Notch, is involved in osteogenesis. Overexpression of Rbpj promoted osteogenic differentiation of mouse Kusa-A1 cells in vitro and in vivo. Transient transfection of an Rbpj expression vector into Kusa-A1 cells upregulated promoter activities of Runx2 and Ose2. Enhanced osteogenic potentials including high alkaline phosphatase activity, rapid calcium deposition, and increased calcified nodule formation, were observed in established stable Rbpj-overexpressing Kusa-A1 (Kusa-A1/Rbpj) cell line. In vivo mineralization by Kusa-A1/Rbpj was promoted compared to that by Kusa-A1 host cells. Histological findings revealed that expression of Rbpj was primarily observed in osteoblasts. These results suggest that Rbpj may play essential roles in osteoblast differentiation.
Volume 400(1)
Pages 39-45
Published 2010-9-10
DOI 10.1016/j.bbrc.2010.07.133
PII S0006-291X(10)01466-X
PMID 20691157
MeSH Animals Calcification, Physiologic / genetics Cell Line Core Binding Factor Alpha 1 Subunit / genetics Gene Expression Regulation, Developmental* Immunoglobulin J Recombination Signal Sequence-Binding Protein / genetics Immunoglobulin J Recombination Signal Sequence-Binding Protein / metabolism* Mesenchymal Stem Cells / cytology* Mice Osteogenesis / genetics* Pluripotent Stem Cells / cytology* Pluripotent Stem Cells / metabolism Promoter Regions, Genetic Receptors, Notch / genetics
IF 2.985
Times Cited 5
DNA material pEF5/FRT/V5/Rbpj (RDB08126) pTag/HA/Rpbj (RDB08127) RBP-J (RDB01801)