論文 - 詳細
| RRC ID | 6863 |
|---|---|
| 著者 | Wang S, Kawashima N, Sakamoto K, Katsube K, Umezawa A, Suda H. |
| タイトル | Osteogenic differentiation of mouse mesenchymal progenitor cell, Kusa-A1 is promoted by mammalian transcriptional repressor Rbpj. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Pluripotent mesenchymal stem cells possess the ability to differentiate into many cell types, but the precise mechanisms of differentiation are still unclear. Here, we provide evidence that Rbpj (recombination signal-binding protein for immunoglobulin kappa j region) protein, the primary nuclear mediator of Notch, is involved in osteogenesis. Overexpression of Rbpj promoted osteogenic differentiation of mouse Kusa-A1 cells in vitro and in vivo. Transient transfection of an Rbpj expression vector into Kusa-A1 cells upregulated promoter activities of Runx2 and Ose2. Enhanced osteogenic potentials including high alkaline phosphatase activity, rapid calcium deposition, and increased calcified nodule formation, were observed in established stable Rbpj-overexpressing Kusa-A1 (Kusa-A1/Rbpj) cell line. In vivo mineralization by Kusa-A1/Rbpj was promoted compared to that by Kusa-A1 host cells. Histological findings revealed that expression of Rbpj was primarily observed in osteoblasts. These results suggest that Rbpj may play essential roles in osteoblast differentiation. |
| 巻・号 | 400(1) |
| ページ | 39-45 |
| 公開日 | 2010-9-10 |
| DOI | 10.1016/j.bbrc.2010.07.133 |
| PII | S0006-291X(10)01466-X |
| PMID | 20691157 |
| MeSH | Animals Calcification, Physiologic / genetics Cell Line Core Binding Factor Alpha 1 Subunit / genetics Gene Expression Regulation, Developmental* Immunoglobulin J Recombination Signal Sequence-Binding Protein / genetics Immunoglobulin J Recombination Signal Sequence-Binding Protein / metabolism* Mesenchymal Stem Cells / cytology* Mice Osteogenesis / genetics* Pluripotent Stem Cells / cytology* Pluripotent Stem Cells / metabolism Promoter Regions, Genetic Receptors, Notch / genetics |
| IF | 2.985 |
| 引用数 | 5 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 遺伝子材料 | pEF5/FRT/V5/Rbpj (RDB08126) pTag/HA/Rpbj (RDB08127) RBP-J (RDB01801) |