RRC ID 6867
Author Cogoi S, Paramasivam M, Membrino A, Yokoyama KK, Xodo LE.
Title The KRAS promoter responds to Myc-associated zinc finger and poly(ADP-ribose) polymerase 1 proteins, which recognize a critical quadruplex-forming GA-element.
Journal J. Biol. Chem.
Abstract A point mutations, KRAS transcription is down-regulated. Conversely, guanidine-modified phthalocyanines, which specifically interact with and stabilize the KRAS G-quadruplex, push the promoter activity up to more than double. Collectively, our data support a transcription mechanism for murine KRAS that involves MAZ, PARP-1 and duplex-quadruplex conformational changes in the promoter GA-element.
Volume 285(29)
Pages 22003-16
Published 2010-7-16
DOI 10.1074/jbc.M110.101923
PII M110.101923
PMID 20457603
PMC PMC2903372
MeSH Animals Base Sequence Chromatin Immunoprecipitation DNA-Binding Proteins / metabolism* Electrophoretic Mobility Shift Assay Fluorescence Resonance Energy Transfer G-Quadruplexes* Guanine / metabolism* Indoles / chemistry Ligands Mice Molecular Sequence Data Mutant Proteins / metabolism NIH 3T3 Cells Nucleic Acid Conformation Poly(ADP-ribose) Polymerases / metabolism* Promoter Regions, Genetic* Protein Binding Protein Stability Proto-Oncogene Proteins p21(ras) / genetics* Transcription Factors / metabolism* Transcription, Genetic
IF 4.011
Times Cited 55
DNA material pGEX-hMAZ (RDB03219) pCMV-MAZ (RDB05701).