RRC ID 68677
Author Zuguchi M, Miki Y, Onodera Y, Fujishima F, Takeyama D, Okamoto H, Miyata G, Sato A, Satomi S, Sasano H.
Title Estrogen receptor α and β in esophageal squamous cell carcinoma.
Journal Cancer Sci
Abstract A gender difference has been reported in the morbidity of esophageal squamous cell carcinoma (ESCC). Estrogens have been proposed to play a role in this difference but the details have not yet been clarified. Therefore, in the present study, we examined the status of estrogen receptor (ER)α and ERβ in 90 Japanese ESCC patients. ERα and ERβ immunoreactivity was detected in the nuclei of ESCC cells (41.1 and 97.8%, respectively). There was a significant positive association between the ERβ H score and histological differentiation (P = 0.0403), TNM-pM (LYM) (P = 0.00164) and Ki67/MIB1 LI of carcinoma cells (P = 0.0497, r = 0.207). In addition, the ERβ status of carcinoma cells was significantly correlated with unfavorable clinical outcome of the patients. Multivariate analysis further revealed the ERβ status in carcinoma cells as an independent unfavorable prognostic factor of these patients. We further examined the effects of estrogen treatment on ESCC cell line (ECGI-10) transfected with ERα or ERβ in vitro. The number of ECGI-10 transfected with ERβ was increased by estradiol or ERβ specific agonist but estradiol did not exert any effect upon the cell number of ECGI-10 transfected with ERα. In summary, the results of the present study clearly demonstrate that the status of ERβ in ESCC was closely associated with the unfavorable prognosis, possibly through altering cell proliferation of carcinoma cells.
Volume 103(7)
Pages 1348-55
Published 2012-7-1
DOI 10.1111/j.1349-7006.2012.02288.x
PMID 22463081
PMC PMC7659275
MeSH Adult Aged Aged, 80 and over Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / metabolism* Carcinoma, Squamous Cell / pathology Cell Line, Tumor Cell Nucleus / metabolism Cell Proliferation / drug effects Esophageal Neoplasms / genetics Esophageal Neoplasms / metabolism* Esophageal Neoplasms / pathology Estradiol / pharmacology Estrogen Receptor alpha / genetics Estrogen Receptor alpha / metabolism* Estrogen Receptor beta / genetics Estrogen Receptor beta / metabolism* Estrogens / pharmacology Female Gene Expression Regulation, Neoplastic Humans Immunohistochemistry Kaplan-Meier Estimate Ki-67 Antigen / metabolism Male Middle Aged Multivariate Analysis Phenols Prognosis Pyrazoles / pharmacology Reverse Transcriptase Polymerase Chain Reaction
IF 4.966
Human and Animal Cells EC-GI-10(RCB0774)