RRC ID 68709
Author Yamasaki M, Motonaga C, Yokoyama M, Ikezaki A, Kakihara T, Hayasegawa R, Yamasaki K, Sakono M, Sakakibara Y, Suiko M, Nishiyama K.
Title Induction of apoptotic cell death in HL-60 cells by jacaranda seed oil derived fatty acids.
Journal J Oleo Sci
Abstract Various fatty acids are attracting considerable interest for their anticancer effects. Among them, fatty acids containing conjugated double bonds show one of the most potent cytotoxic effects on cancer cells. Here, we focused on the cancer cell killing activity of jacaranda seed oil. The seed oil of jacaranda harvested from Miyazaki in Japan contained 30.9% cis-8, trans-10, cis-12 octadecatrienoic acid, called jacaric acid (JA). Fatty acid prepared from this oil (JFA) and JA strongly induced cell death in human leukemia HL-60 cells. On the other hand, linoleic acid and trans-10, cis-12 conjugated linoleic acid (<10 μM) did not affect cell proliferation and viability. An increase in the sub-G₁ population and internucleosomal fragmentation of DNA was observed in JA- and JFA-treated cells, indicating induction of apoptotic cell death. Finally, the cytotoxic effects of JA and JFA were completely abolished by α-tocopherol. Taken together, these data suggest that jacaranda seed oil has potent apoptotic activity in HL-60 cells through induction of oxidative stress.
Volume 62(11)
Pages 925-32
Published 2013-1-1
DOI 10.5650/jos.62.925
PII DN/JST.JSTAGE/jos/62.925
PMID 24200941
MeSH Antineoplastic Agents, Phytogenic / chemistry Antineoplastic Agents, Phytogenic / pharmacology* Apoptosis / drug effects* Apoptosis / genetics DNA Fragmentation / drug effects G1 Phase / drug effects HL-60 Cells Humans Linolenic Acids / chemistry Linolenic Acids / isolation & purification Linolenic Acids / pharmacology* Nucleosomes / genetics Oxidative Stress / drug effects Plant Oils / chemistry* Plant Oils / pharmacology Seeds / chemistry Structure-Activity Relationship alpha-Tocopherol / pharmacology
IF 1.304
Human and Animal Cells HL60(RCB0041)