RRC ID 68728
Author Chang DJ, Lee N, Park IH, Choi C, Jeon I, Kwon J, Oh SH, Shin DA, Do JT, Lee DR, Lee H, Moon H, Hong KS, Daley GQ, Song J.
Title Therapeutic potential of human induced pluripotent stem cells in experimental stroke.
Journal Cell Transplant
Abstract Ischemic stroke mainly caused by middle cerebral artery occlusion (MCAo) is a major type of stroke, but there are currently very limited therapeutic options for its cure. Neural stem cells (NSCs) or neural precursor cells (NPCs) derived from various sources are known to survive and improve neurological functions when they are engrafted in animal models of stroke. Induced pluripotent stem cells (iPSCs) generated from somatic cells of patients are novel cells that promise the autologous cell therapy for stroke. In this study, we successfully differentiated iPSCs derived from human fibroblasts into NPCs and found their robust therapeutic potential in a rodent MCAo stroke model. We observed the significant graft-induced behavioral recovery, as well as extensive neural tissue formation. Animal MRI results indicated that the majority of contralaterally transplanted iPSC-derived NPCs migrated to the peri-infarct area, showing a pathotropism critical for tissue recovery. The transplanted animals exhibited the significant reduction of stroke-induced inflammatory response, gliosis and apoptosis, and the contribution to the endogenous neurogenesis. Our results demonstrate that iPSC-derived NPCs are effective cells for the treatment of stroke.
Volume 22(8)
Pages 1427-40
Published 2013-1-1
DOI 10.3727/096368912X657314
PII ct0833chang
PMID 23044029
MeSH Animals Apoptosis Behavior, Animal Cell Differentiation Cell Line Cell Tracking Disease Models, Animal Gliosis / pathology Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / transplantation* Infarction, Middle Cerebral Artery / complications Infarction, Middle Cerebral Artery / pathology Inflammation / pathology Magnetic Resonance Imaging Male Mice Neural Stem Cells / cytology Neural Stem Cells / transplantation Neurogenesis Neurons / cytology Rats Rats, Sprague-Dawley Stem Cell Transplantation* Stroke / etiology Stroke / pathology Stroke / therapy*
IF 3.341
Human and Animal Cells MC3T3-G2/PA6(RCB1127)