RRC ID 68741
Author Ono T, Yamashita D, Hirano T.
Title Condensin II initiates sister chromatid resolution during S phase.
Journal J Cell Biol
Abstract Condensins I and II are multisubunit complexes that play essential yet distinct functions in chromosome condensation and segregation in mitosis. Unlike condensin I, condensin II localizes to the nucleus during interphase, but it remains poorly understood what functions condensin II might have before mitotic entry. Here, we report that condensin II changes its chromatin-binding property during S phase. Remarkably, advanced premature chromosome condensation (PCC) assays enabled us to visualize condensin II forming "sister axes" in replicated regions of chromosomes in S phase cells. Depletion of condensin II compromised PCC-driven sister chromatid resolution during S phase. Moreover, fluorescence in situ hybridization assays revealed that condensin II, but not condensin I, promotes disjoining duplicated chromosomal loci during S phase. Application of mild replicative stress partially impaired this process and further exacerbated phenotypes arising from condensin II depletion. Our results suggest that condensin II initiates structural reorganization of duplicated chromosomes during S phase to prepare for their proper condensation and segregation in mitosis.
Volume 200(4)
Pages 429-41
Published 2013-2-18
DOI 10.1083/jcb.201208008
PII jcb.201208008
PMID 23401001
PMC PMC3575537
MeSH Adenosine Triphosphatases / metabolism Adenosine Triphosphatases / physiology* Chromatids / metabolism* DNA Replication DNA-Binding Proteins / metabolism DNA-Binding Proteins / physiology* HeLa Cells Humans In Situ Hybridization, Fluorescence Multiprotein Complexes / metabolism Multiprotein Complexes / physiology* S Phase* Stress, Physiological
IF 8.811
Human and Animal Cells HeLa(RCB0007)