| RRC ID |
68753
|
| Author |
Wang X, Li X, Onuma K, Sogo Y, Ohno T, Ito A.
|
| Title |
Zn- and Mg- containing tricalcium phosphates-based adjuvants for cancer immunotherapy.
|
| Journal |
Sci Rep
|
| Abstract |
Zn-, and Mg-containing tricalcium phosphates (TCPs) loaded with a hydrothermal extract of a human tubercle bacillus (HTB) were prepared by immersing Zn-TCP and Mg-TCP in HTB-containing supersaturated calcium phosphate solutions. The in vitro and in vivo immunogenic activities of the HTB-loaded Zn-, and Mg-TCPs (Zn-Ap-HTB and Mg-Ap-HTB, respectively) were evaluated as potential immunopotentiating adjuvants for cancer immunotherapy. The Zn-Ap-HTB and Mg-Ap-HTB adjuvants showed no obvious cytotoxicity and more effectively stimulated granulocyte-macrophage colony-stimulating factor (GM-CSF) secretion by macrophage-like cells than unprocessed HTB or HTB-loaded TCP (T-Ap-HTB) in vitro. Zn-Ap-HTB and Mg-Ap-HTB mixed with liquid-nitrogen-treated tumor tissue markedly inhibited the in vivo development of rechallenged Lewis lung carcinoma (LLC) cells compared with T-Ap-HTB and the unprocessed HTB mixed liquid-nitrogen-treated tumor tissue. Zn-Ap-HTB and Mg-Ap-HTB contributed to eliciting potent systemic antitumor immunity in vivo.
|
| Volume |
3
|
| Pages |
2203
|
| Published |
2013-1-1
|
| DOI |
10.1038/srep02203
|
| PII |
srep02203
|
| PMID |
23857555
|
| PMC |
PMC3712317
|
| MeSH |
Adjuvants, Immunologic*
Animals
Bacillus / chemistry
Bacillus / immunology
Calcium Phosphates / administration & dosage
Calcium Phosphates / chemistry*
Calcium Phosphates / immunology*
Calcium Phosphates / toxicity
Cancer Vaccines* / administration & dosage
Cancer Vaccines* / chemistry
Cancer Vaccines* / immunology
Cancer Vaccines* / toxicity
Cytokines / biosynthesis
Disease Models, Animal
Female
Granulocyte-Macrophage Colony-Stimulating Factor / biosynthesis
Humans
Immunotherapy*
Macrophages / immunology
Macrophages / metabolism
Magnesium / chemistry
Mice
Neoplasms / immunology*
Neoplasms / mortality
Neoplasms / therapy*
Spleen / immunology
Spleen / metabolism
Vaccines, Conjugate
Zinc / chemistry
|
| IF |
3.998
|
| Resource |
| Human and Animal Cells |
THP-1(RCB1189)
LLC(RCB0558) |
