| RRC ID |
68767
|
| Author |
Yaguchi A, Oshikawa M, Watanabe G, Hiramatsu H, Uchida N, Hara C, Kaneko N, Sawamoto K, Muraoka T, Ajioka I.
|
| Title |
Efficient protein incorporation and release by a jigsaw-shaped self-assembling peptide hydrogel for injured brain regeneration.
|
| Journal |
Nat Commun
|
| Abstract |
During injured tissue regeneration, the extracellular matrix plays a key role in controlling and coordinating various cellular events by binding and releasing secreted proteins in addition to promoting cell adhesion. Herein, we develop a cell-adhesive fiber-forming peptide that mimics the jigsaw-shaped hydrophobic surface in the dovetail-packing motif of glycophorin A as an artificial extracellular matrix for regenerative therapy. We show that the jigsaw-shaped self-assembling peptide forms several-micrometer-long supramolecular nanofibers through a helix-to-strand transition to afford a hydrogel under physiological conditions and disperses homogeneously in the hydrogel. The molecular- and macro-scale supramolecular properties of the jigsaw-shaped self-assembling peptide hydrogel allow efficient incorporation and sustained release of vascular endothelial growth factor, and demonstrate cell transplantation-free regenerative therapeutic effects in a subacute-chronic phase mouse stroke model. This research highlights a therapeutic strategy for injured tissue regeneration using the jigsaw-shaped self-assembling peptide supramolecular hydrogel.
|
| Volume |
12(1)
|
| Pages |
6623
|
| Published |
2021-11-19
|
| DOI |
10.1038/s41467-021-26896-3
|
| PII |
10.1038/s41467-021-26896-3
|
| PMID |
34799548
|
| PMC |
PMC8604910
|
| MeSH |
Adhesives
Animals
Biomedical Engineering
Brain Injuries / diagnostic imaging
Brain Regeneration / physiology*
Cell Adhesion
Disease Models, Animal
Female
Green Fluorescent Proteins / chemistry
Hydrogels / chemistry*
Hydrogels / therapeutic use
Hydrophobic and Hydrophilic Interactions
Mice
Mice, Inbred C57BL
Nanofibers
Nervous System
Peptides / chemistry*
Peptides / therapeutic use
Proteins / chemistry*
Vascular Endothelial Growth Factor A
|
| IF |
12.121
|
| Resource |
| Human and Animal Cells |
293T(RCB2202) |
