RRC ID 68790
著者 Hacisuleyman E, Goff LA, Trapnell C, Williams A, Henao-Mejia J, Sun L, McClanahan P, Hendrickson DG, Sauvageau M, Kelley DR, Morse M, Engreitz J, Lander ES, Guttman M, Lodish HF, Flavell R, Raj A, Rinn JL.
タイトル Topological organization of multichromosomal regions by the long intergenic noncoding RNA Firre.
ジャーナル Nat Struct Mol Biol
Abstract RNA, including long noncoding RNA (lncRNA), is known to be an abundant and important structural component of the nuclear matrix. However, the molecular identities, functional roles and localization dynamics of lncRNAs that influence nuclear architecture remain poorly understood. Here, we describe one lncRNA, Firre, that interacts with the nuclear-matrix factor hnRNPU through a 156-bp repeating sequence and localizes across an ~5-Mb domain on the X chromosome. We further observed Firre localization across five distinct trans-chromosomal loci, which reside in spatial proximity to the Firre genomic locus on the X chromosome. Both genetic deletion of the Firre locus and knockdown of hnRNPU resulted in loss of colocalization of these trans-chromosomal interacting loci. Thus, our data suggest a model in which lncRNAs such as Firre can interface with and modulate nuclear architecture across chromosomes.
巻・号 21(2)
ページ 198-206
公開日 2014-2-1
DOI 10.1038/nsmb.2764
PII nsmb.2764
PMID 24463464
PMC PMC3950333
MeSH Animals Base Sequence Chromatin / metabolism Chromosomes / metabolism* Chromosomes / ultrastructure Embryonic Stem Cells Female Humans Male Mice Models, Genetic* Molecular Sequence Data RNA, Long Noncoding / analysis RNA, Long Noncoding / chemistry RNA, Long Noncoding / physiology* Sequence Analysis, RNA X Chromosome Inactivation
IF 11.98
リソース情報
ヒト・動物細胞 BRC6(AES0010)