論文 - 詳細
| RRC ID | 68816 |
|---|---|
| 著者 | Ohashi S, Kikuchi O, Tsurumaki M, Nakai Y, Kasai H, Horimatsu T, Miyamoto S, Shimizu A, Chiba T, Muto M. |
| タイトル | Preclinical validation of talaporfin sodium-mediated photodynamic therapy for esophageal squamous cell carcinoma. |
| ジャーナル | PLoS One |
| Abstract |
Photodynamic therapy (PDT) kills cancer cells via a photochemical reaction mediated by an oncotropic photosensitizer. Herein, we performed an experimental preclinical study to validate the anti-tumour effect of talaporfin sodium-mediated PDT (t-PDT) for esophageal squamous cell carcinoma (ESCC) cells. We used human ESCC cells derived from various differentiation grades or resistant to 5-fluorouracil (5-FU). The cytotoxic effect of t-PDT was determined by evaluating cell viability, apoptosis and generation of reactive oxygen species (ROS) and DNA double-strand breaks. Furthermore, the anti-tumour effect of t-PDT was assessed using an anchorage-independent cell-growth assay and xenograft transplantation models. t-PDT induced potent cytotoxicity in ESCC cells independent of their differentiation grade or 5-FU resistance. Moreover, t-PDT induced robust apoptosis, as indicated by cell shrinkage, perinuclear vacuolization, nuclear fragmentation and induction of annexin V-positive cells. This apoptotic response was accompanied by concurrent activation of ROS, and induction of DNA double-strand breakage. Importantly, t-PDT suppressed efficiently anchorage-independent cell growth as well as ESCC-xenografted tumor formation. In aggregate, t-PDT showed anti-tumor potential for ESCC cells with various histological grades or chemoresistance, providing a novel translational rationale of t-PDT for the treatment of ESCC. |
| 巻・号 | 9(8) |
| ページ | e103126 |
| 公開日 | 2014-1-1 |
| DOI | 10.1371/journal.pone.0103126 |
| PII | PONE-D-14-15434 |
| PMID | 25090101 |
| PMC | PMC4121166 |
| MeSH | Animals Apoptosis / drug effects Carcinoma, Squamous Cell / drug therapy* Carcinoma, Squamous Cell / pathology Cell Adhesion / drug effects Cell Line, Tumor Cell Proliferation / drug effects DNA Breaks, Double-Stranded / drug effects Esophageal Neoplasms / drug therapy* Esophageal Neoplasms / pathology Esophageal Squamous Cell Carcinoma Fluorescence Humans Intracellular Space / drug effects Intracellular Space / metabolism Mice Photochemotherapy* Porphyrins / pharmacology Porphyrins / therapeutic use* Reactive Oxygen Species / metabolism Reproducibility of Results |
| IF | 2.74 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | TE-5(RCB1949) TE-8(RCB2098) TE-10(RCB2099) TE-11(RCB2100) |