| RRC ID |
68816
|
| 著者 |
Ohashi S, Kikuchi O, Tsurumaki M, Nakai Y, Kasai H, Horimatsu T, Miyamoto S, Shimizu A, Chiba T, Muto M.
|
| タイトル |
Preclinical validation of talaporfin sodium-mediated photodynamic therapy for esophageal squamous cell carcinoma.
|
| ジャーナル |
PLoS One
|
| Abstract |
Photodynamic therapy (PDT) kills cancer cells via a photochemical reaction mediated by an oncotropic photosensitizer. Herein, we performed an experimental preclinical study to validate the anti-tumour effect of talaporfin sodium-mediated PDT (t-PDT) for esophageal squamous cell carcinoma (ESCC) cells. We used human ESCC cells derived from various differentiation grades or resistant to 5-fluorouracil (5-FU). The cytotoxic effect of t-PDT was determined by evaluating cell viability, apoptosis and generation of reactive oxygen species (ROS) and DNA double-strand breaks. Furthermore, the anti-tumour effect of t-PDT was assessed using an anchorage-independent cell-growth assay and xenograft transplantation models. t-PDT induced potent cytotoxicity in ESCC cells independent of their differentiation grade or 5-FU resistance. Moreover, t-PDT induced robust apoptosis, as indicated by cell shrinkage, perinuclear vacuolization, nuclear fragmentation and induction of annexin V-positive cells. This apoptotic response was accompanied by concurrent activation of ROS, and induction of DNA double-strand breakage. Importantly, t-PDT suppressed efficiently anchorage-independent cell growth as well as ESCC-xenografted tumor formation. In aggregate, t-PDT showed anti-tumor potential for ESCC cells with various histological grades or chemoresistance, providing a novel translational rationale of t-PDT for the treatment of ESCC.
|
| 巻・号 |
9(8)
|
| ページ |
e103126
|
| 公開日 |
2014-1-1
|
| DOI |
10.1371/journal.pone.0103126
|
| PII |
PONE-D-14-15434
|
| PMID |
25090101
|
| PMC |
PMC4121166
|
| MeSH |
Animals
Apoptosis / drug effects
Carcinoma, Squamous Cell / drug therapy*
Carcinoma, Squamous Cell / pathology
Cell Adhesion / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects
DNA Breaks, Double-Stranded / drug effects
Esophageal Neoplasms / drug therapy*
Esophageal Neoplasms / pathology
Esophageal Squamous Cell Carcinoma
Fluorescence
Humans
Intracellular Space / drug effects
Intracellular Space / metabolism
Mice
Photochemotherapy*
Porphyrins / pharmacology
Porphyrins / therapeutic use*
Reactive Oxygen Species / metabolism
Reproducibility of Results
|
| IF |
2.74
|
| リソース情報 |
| ヒト・動物細胞 |
TE-5(RCB1949)
TE-8(RCB2098)
TE-10(RCB2099)
TE-11(RCB2100) |
