論文 - 詳細
| RRC ID | 68882 |
|---|---|
| 著者 | Daikuzono H, Yamazaki M, Sato Y, Takahashi T, Yamagata K. |
| タイトル | Development of a DELFIA method to detect oncofetal antigen ROR1-positive exosomes. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Receptor tyrosine kinase-like orphan receptor 1 (ROR1) is highly expressed in a wide variety of hematological and solid cancers, but is low or absent in adult tissues. Here, we show that ROR1 is released with exosomes from ROR1-positive cancer cells. We also developed a simple dissociation-enhanced lanthanide fluorescence immunoassay (DELFIA) to detect cancer-derived ROR1-positive exosomes, which are captured by two anti-ROR1 antibodies and detected by the fluorescence of free chelating europium. This new DELFIA method can detect cancer-derived ROR1-positive exosomes in the cell supernatant and serum with a wide range and rapidly compared with the conventional Western blot assay. This method may be useful as a companion diagnostics for ROR1-positive cancers. |
| 巻・号 | 578 |
| ページ | 170-176 |
| 公開日 | 2021-11-12 |
| DOI | 10.1016/j.bbrc.2021.08.054 |
| PII | S0006-291X(21)01237-7 |
| PMID | 34597914 |
| MeSH | Animals Antigens, Neoplasm / metabolism* Biomarkers, Tumor / blood* Biomarkers, Tumor / metabolism Cell Line, Tumor Disease Models, Animal Exosomes / metabolism Exosomes / pathology* Female Humans Immunoassay / methods* Mice Mice, Inbred BALB C Neoplasms / blood Neoplasms / metabolism Neoplasms / pathology* Receptor Tyrosine Kinase-like Orphan Receptors / metabolism* |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | DU145(RCB2143) PANC-1(RCB2095) MIA Paca2(RCB2094) |