RRC ID |
68882
|
Author |
Daikuzono H, Yamazaki M, Sato Y, Takahashi T, Yamagata K.
|
Title |
Development of a DELFIA method to detect oncofetal antigen ROR1-positive exosomes.
|
Journal |
Biochem Biophys Res Commun
|
Abstract |
Receptor tyrosine kinase-like orphan receptor 1 (ROR1) is highly expressed in a wide variety of hematological and solid cancers, but is low or absent in adult tissues. Here, we show that ROR1 is released with exosomes from ROR1-positive cancer cells. We also developed a simple dissociation-enhanced lanthanide fluorescence immunoassay (DELFIA) to detect cancer-derived ROR1-positive exosomes, which are captured by two anti-ROR1 antibodies and detected by the fluorescence of free chelating europium. This new DELFIA method can detect cancer-derived ROR1-positive exosomes in the cell supernatant and serum with a wide range and rapidly compared with the conventional Western blot assay. This method may be useful as a companion diagnostics for ROR1-positive cancers.
|
Volume |
578
|
Pages |
170-176
|
Published |
2021-11-12
|
DOI |
10.1016/j.bbrc.2021.08.054
|
PII |
S0006-291X(21)01237-7
|
PMID |
34597914
|
MeSH |
Animals
Antigens, Neoplasm / metabolism*
Biomarkers, Tumor / blood*
Biomarkers, Tumor / metabolism
Cell Line, Tumor
Disease Models, Animal
Exosomes / metabolism
Exosomes / pathology*
Female
Humans
Immunoassay / methods*
Mice
Mice, Inbred BALB C
Neoplasms / blood
Neoplasms / metabolism
Neoplasms / pathology*
Receptor Tyrosine Kinase-like Orphan Receptors / metabolism*
|
IF |
2.985
|
Resource |
Human and Animal Cells |
DU145(RCB2143)
PANC-1(RCB2095)
MIA Paca2(RCB2094) |