| RRC ID |
68897
|
| Author |
Terashima M, Amano M, Onodera T, Nishimura S, Iwasaki N.
|
| Title |
Quantitative glycomics monitoring of induced pluripotent- and embryonic stem cells during neuronal differentiation.
|
| Journal |
Stem Cell Res
|
| Abstract |
Alterations in the structure of cell surface glycoforms occurring during the stages of stem cell differentiation remain unclear. We describe a rapid glycoblotting-based cellular glycomics method for quantitatively evaluating changes in glycoform expression and structure during neuronal differentiation of murine induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs). Our results show that changes in the expression of cellular N-glycans are comparable during the differentiation of iPSCs and ESCs. The expression of bisect-type N-glycans was significantly up-regulated in neurons that differentiated from both iPSCs and ESCs. From a glycobiological standpoint, iPSCs are an alternative neural cell source in addition to ESCs.
|
| Volume |
13(3 Pt A)
|
| Pages |
454-64
|
| Published |
2014-11-1
|
| DOI |
10.1016/j.scr.2014.10.006
|
| PII |
S1873-5061(14)00116-0
|
| PMID |
25460606
|
| MeSH |
Animals
Cell Differentiation
Cells, Cultured
Cerebral Cortex / cytology
Cerebral Cortex / metabolism
Embryonic Stem Cells / cytology
Embryonic Stem Cells / metabolism*
Glycomics*
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / metabolism*
Mice
Mice, Inbred C57BL
N-Acetylglucosaminyltransferases / genetics
N-Acetylglucosaminyltransferases / metabolism
Nestin / genetics
Nestin / metabolism
Neurons / cytology
Neurons / metabolism*
Polysaccharides / metabolism*
RNA, Messenger / metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
|
| IF |
4.495
|
| Resource |
| Human and Animal Cells |
mouse iPSCs
mouse ESC |
