論文 - 詳細
| RRC ID | 68900 |
|---|---|
| 著者 | Junedi S, Yasuhara K, Nagao S, Kikuchi J, Hirota S. |
| タイトル | Morphological change of cell membrane by interaction with domain-swapped cytochrome c oligomers. |
| ジャーナル | Chembiochem |
| Abstract |
Monomeric cyt c has been reported to bind to the mitochondrial membrane by electrostatic and hydrophobic interactions with anionic phospholipids. We have previously shown that domain-swapped oligomeric cyt c retains the secondary structure of the monomer, and its surface possesses a larger area and more charges compared to the monomer. However, the effect of oligomerization of cyt c on cells has yet to be revealed. Herein, we investigated the interaction of oligomeric cyt c with anionic phospholipid-containing vesicles and the outer membrane of HeLa cells. Oligomeric cyt c interacted more strongly than monomeric cyt c with anionic phospholipid-containing vesicles and the outer membrane of HeLa cells. Oligomeric cyt c induced lateral phase separation of lipids in LUVs and GUVs, thereby leading to membrane disruption, whereas monomeric cyt c did not. Morphological changes in HeLa cells resulted from interaction with oligomeric cyt c, but little from interaction with the monomer. These results show that domain-swapped oligomeric proteins might exhibit properties different to those of monomer in cell systems. |
| 巻・号 | 15(4) |
| ページ | 517-21 |
| 公開日 | 2014-3-3 |
| DOI | 10.1002/cbic.201300728 |
| PMID | 24449526 |
| MeSH | Animals Cell Membrane / chemistry Cell Membrane / metabolism* Cell Shape Cytochromes c / chemistry Cytochromes c / metabolism* HeLa Cells Horses Humans Lipid Bilayers / chemistry Lipid Bilayers / metabolism Liposomes / chemistry Liposomes / metabolism Phospholipids / chemistry Protein Multimerization Protein Structure, Tertiary |
| IF | 2.576 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |