論文 - 詳細
| RRC ID | 68902 |
|---|---|
| 著者 | Nakase I, Osaki K, Tanaka G, Utani A, Futaki S. |
| タイトル | Molecular interplays involved in the cellular uptake of octaarginine on cell surfaces and the importance of syndecan-4 cytoplasmic V domain for the activation of protein kinase Cα. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Arginine-rich cell-penetrating peptides (CPPs) are promising carriers for the intracellular delivery of various bioactive molecules. However, many ambiguities remain about the molecular interplays on cell surfaces that ultimately lead to endocytic uptake of CPPs. By treatment of cells with octaarginine (R8), enhanced clustering of syndecan-4 on plasma membranes and binding of protein kinase Cα (PKCα) to the cytoplasmic domain of syndecan-4 were observed; these events potentially lead to the macropinocytic uptake of R8. The cytoplasmic V domain of syndecan-4 made a significant contribution to the cellular uptake of R8, whereas the cytoplasmic C1 and C2 domains were not involved in the process. |
| 巻・号 | 446(4) |
| ページ | 857-62 |
| 公開日 | 2014-4-18 |
| DOI | 10.1016/j.bbrc.2014.03.018 |
| PII | S0006-291X(14)00449-5 |
| PMID | 24632200 |
| MeSH | Cell Membrane / metabolism* Cell-Penetrating Peptides / analysis Cell-Penetrating Peptides / metabolism* Endocytosis Enzyme Activation HeLa Cells Humans Oligopeptides / analysis Oligopeptides / metabolism* Pinocytosis Protein Binding Protein Kinase C-alpha / analysis Protein Kinase C-alpha / metabolism* Protein Structure, Tertiary Syndecan-4 / analysis Syndecan-4 / metabolism* |
| IF | 2.985 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa |