論文 - 詳細
| RRC ID | 68927 |
|---|---|
| 著者 | Hiyoshi M, Takahashi N, Eltalkhawy YM, Noyori O, Lotfi S, Panaampon J, Okada S, Tanaka Y, Ueno T, Fujisawa JI, Sato Y, Suzuki T, Hasegawa H, Tokunaga M, Satou Y, Yasunaga JI, Matsuoka M, Utsunomiya A, Suzu S. |
| タイトル | M-Sec induced by HTLV-1 mediates an efficient viral transmission. |
| ジャーナル | PLoS Pathog |
| Abstract |
Human T-cell leukemia virus type 1 (HTLV-1) infects target cells primarily through cell-to-cell routes. Here, we provide evidence that cellular protein M-Sec plays a critical role in this process. When purified and briefly cultured, CD4+ T cells of HTLV-1 carriers, but not of HTLV-1- individuals, expressed M-Sec. The viral protein Tax was revealed to mediate M-Sec induction. Knockdown or pharmacological inhibition of M-Sec reduced viral infection in multiple co-culture conditions. Furthermore, M-Sec knockdown reduced the number of proviral copies in the tissues of a mouse model of HTLV-1 infection. Phenotypically, M-Sec knockdown or inhibition reduced not only plasma membrane protrusions and migratory activity of cells, but also large clusters of Gag, a viral structural protein required for the formation of viral particles. Taken together, these results suggest that M-Sec induced by Tax mediates an efficient cell-to-cell viral infection, which is likely due to enhanced membrane protrusions, cell migration, and the clustering of Gag. |
| 巻・号 | 17(11) |
| ページ | e1010126 |
| 公開日 | 2021-11-1 |
| DOI | 10.1371/journal.ppat.1010126 |
| PII | PPATHOGENS-D-21-01736 |
| PMID | 34843591 |
| PMC | PMC8659635 |
| MeSH | Animals Cell Membrane / metabolism Cell Membrane / virology* Cell Movement Coculture Techniques Disease Models, Animal* Gene Products, tax / genetics Gene Products, tax / metabolism* HTLV-I Infections / metabolism HTLV-I Infections / transmission* HTLV-I Infections / virology Human T-lymphotropic virus 1 / physiology* Humans Mice Mice, Inbred NOD Mice, SCID Tumor Necrosis Factors / genetics Tumor Necrosis Factors / metabolism* Viral Structural Proteins / genetics Viral Structural Proteins / metabolism* |
| IF | 6.218 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |