論文 - 詳細
| RRC ID | 68928 |
|---|---|
| 著者 | Yamaki K, Terashi M, Yamamoto S, Fujiwara R, Inoue JI, Shimizu K, Yanagita S, Doi Y, Kimura KI, Kotani K, Sugihara M, Koyama Y. |
| タイトル | Immunoglobulin a suppresses B cell receptor-mediated activation of mouse B cells with differential inhibition of signaling molecules. |
| ジャーナル | Immunopharmacol Immunotoxicol |
| Abstract |
CONTEXT:We previously reported that monoclonal mouse immunoglobulin (Ig) A, OA-4, attenuates sensitization in mice by suppressing B cell activation. OBJECTIVE:Here, it is demonstrated for the first time that mouse IgA inhibits mouse B cell activation in vitro under natural conditions (i.e. in the absence of chemical, physical, and genetic modifications of IgA and B cells). MATERIALS AND METHODS:Mouse splenocytes were stimulated with anti-B cell receptor (BCR) antibody or lipopolysaccharide (LPS) in the presence or absence of OA-4. Splenic B cell proliferation and the activation of several intracellular signaling molecules were measured. RESULTS:Anti-BCR antibody-induced proliferation was markedly inhibited by OA-4 or the commercially available mouse IgA S107, whereas LPS-induced proliferation was weakly attenuated by a high concentration of OA-4. Moreover, OA-4 markedly decreased the anti-BCR antibody-induced phosphorylation of p44/42 mitogen-activated protein kinase (ERK) and CD22 and decreased phosphorylated phospholipase (PLC) γ2 and intracellular Ca2+ levels moderately, whereas protein kinase B (Akt) phosphorylation was not affected by OA-4. The MAPK/ERK kinase-ERK and phosphoinositide 3-kinase-Akt pathways were found to play a role in the proliferation of splenocytes induced by anti-BCR antibody based on experiments with their inhibitors. In contrast to that in splenic B cells, ERK phosphorylation induced by anti-BCR antibody in A20 cells was not inhibited by OA-4. The modulatory effects of IgA were different among the cell types and signaling pathways. CONCLUSION:IgA is a potential immunoregulatory drug utilizing new mechanisms that affect splenic B cells but not A20 lymphomas. |
| 巻・号 | 44(1) |
| ページ | 76-86 |
| 公開日 | 2022-2-1 |
| DOI | 10.1080/08923973.2021.2006216 |
| PMID | 34844505 |
| MeSH | Animals B-Lymphocytes / immunology* Immunoglobulin A* Lymphocyte Activation Mice Phosphatidylinositol 3-Kinases / metabolism Phosphorylation Receptors, Antigen, B-Cell* / metabolism Signal Transduction* |
| IF | 2.352 |
| リソース情報 | |
| ヒト・動物細胞 | A20(RCB2745) |