RRC ID 68938
著者 Shimizu K, Iyoda T, Sanpei A, Nakazato H, Okada M, Ueda S, Kato-Murayama M, Murayama K, Shirouzu M, Harada N, Hidaka M, Fujii SI.
タイトル Identification of TCR repertoires in functionally competent cytotoxic T cells cross-reactive to SARS-CoV-2.
ジャーナル Commun Biol
Abstract SARS-CoV-2-specific CD8+ T cells are scarce but detectable in unexposed healthy donors (UHDs). It remains unclear whether pre-existing human coronavirus (HCoV)-specific CD8+ T cells are converted to functionally competent T cells cross-reactive to SARS-CoV-2. Here, we identified the HLA-A24-high binding, immunodominant epitopes in SARS-CoV-2 spike region that can be recognized by seasonal coronavirus-specific CD8+ T cells from HLA-A24+ UHDs. Cross-reactive CD8+ T cells were clearly reduced in patients with hematological malignancy, who are usually immunosuppressed, compared to those in UHDs. Furthermore, we showed that CD8+ T cells in response to a selected dominant epitope display multifunctionality and cross-functionality across HCoVs in HLA-A24+ donors. Cross-reactivity of T-cell receptors isolated from them exhibited selective diversity at the single-cell level. Taken together, when stimulated well by immunodominant epitopes, selective pre-existing CD8+ T cells with high functional avidity may be cross-reactive against SARS-CoV-2.
巻・号 4(1)
ページ 1365
公開日 2021-12-2
DOI 10.1038/s42003-021-02885-6
PII 10.1038/s42003-021-02885-6
PMID 34857854
PMC PMC8640030
MeSH Antigens, Viral / immunology* CD8-Positive T-Lymphocytes / immunology COVID-19 / immunology Cross Reactions Humans Immunodominant Epitopes / immunology* Receptors, Antigen, T-Cell / immunology* SARS-CoV-2 / immunology* T-Lymphocytes, Cytotoxic / immunology*
IF 4.165
リソース情報
ヒト・動物細胞 SKW-3(RCB1168)