| RRC ID |
68961
|
| 著者 |
Takamatsu K, Tanaka J, Katada R, Azuma K, Takakura I, Aota K, Kamatani T, Shirota T, Inoue S, Mishima K.
|
| タイトル |
Aging-associated stem/progenitor cell dysfunction in the salivary glands of mice.
|
| ジャーナル |
Exp Cell Res
|
| Abstract |
Although stem cell aging leads to a decline in tissue homeostasis and regenerative capacity, it remains unclear whether salivary gland stem cell function changes during this process. However, the salivary glands are gradually replaced by connective tissue during aging. Here, we show a decline in the stem cell ability of CD133-positive stem/progenitor cells in the salivary glands of aged mice. The CD133-positive cells were isolated from young, adult, and aged mice. The number of CD133-positive cells was significantly decreased in aged mice. They also showed a lower sphere formation capacity compared to young and adult mice. RNA sequencing revealed that CD133-positive cells in aged mice exhibited lower gene expression of several aging-related genes, including FoxO3a, than those in young and adult mice. Salivary gland cells infected with a recombinant lentivirus encoding the FoxO3a gene showed a reduction in oxidative stress induced by hydrogen peroxide compared with those infected with a control virus. Thus, FoxO3a may inhibit stem cell aging via oxidative stress.
|
| 巻・号 |
409(1)
|
| ページ |
112889
|
| 公開日 |
2021-12-1
|
| DOI |
10.1016/j.yexcr.2021.112889
|
| PII |
S0014-4827(21)00443-2
|
| PMID |
34678306
|
| MeSH |
Aging / pathology*
Animals
Cell Line
Cellular Senescence / physiology*
Female
HEK293 Cells
Humans
Mice
Mice, Inbred C57BL
Regeneration / physiology
Salivary Glands / pathology*
Stem Cell Transplantation / methods
Stem Cells / pathology*
|
| IF |
3.383
|
| リソース情報 |
| 遺伝子材料 |
Genome Network Project Human cDNA IRAK168O23 (HGY067559) |
