RRC ID 69030
著者 Tadokoro S, Tokuyama-Toda R, Tatehara S, Ide S, Umeki H, Miyoshi K, Noma T, Satomura K.
タイトル A New Induction Method for the Controlled Differentiation of Human-Induced Pluripotent Stem Cells Using Frozen Sections.
ジャーナル Cells
Abstract Considering that every tissue/organ has the most suitable microenvironment for its functional cells, controlling induced pluripotent stem cell (iPSC) differentiation by culture on frozen sections having a suitable microenvironment is possible. Induced PSCs were cultured on frozen sections of the liver, the brain, the spinal cord, and cover glasses (control) for 9 days. The iPSCs cultured on the sections of the liver resembled hepatocytes, whereas those on sections of the brain and the spinal cord resembled neuronal cells. The percentage of hepatocytic marker-positive cells in the iPSCs cultured on the sections of the liver was statistically higher than that of those in the iPSCs cultured on the sections of the brain and the spinal cord or on cover glasses. In contrast, the iPSCs cultured on the sections of the brain and the spinal cord revealed a high percentage of neural marker-positive cells. Thus, iPSCs can be differentiated into a specific cell lineage in response to specific factors within frozen sections of tissues/organs. Differentiation efficacy of the frozen sections markedly differed between the iPSC clones. Therefore, our induction method could be simple and effective for evaluating the iPSC quality.
巻・号 10(11)
公開日 2021-10-21
DOI 10.3390/cells10112827
PII cells10112827
PMID 34831050
PMC PMC8616214
MeSH Animals Biomarkers / metabolism Cell Differentiation* Cells, Cultured Frozen Sections / methods* Hepatocytes / cytology Hepatocytes / metabolism Humans Induced Pluripotent Stem Cells / cytology* Male Mice, Inbred ICR
IF 4.366
リソース情報
ヒト・動物細胞 201B7(HPS0063) 409B2(HPS0076) 454E2(HPS0077)