RRC ID 69153
Author Thyer R, d'Oelsnitz S, Blevins MS, Klein DR, Brodbelt JS, Ellington AD.
Title Directed Evolution of an Improved Aminoacyl-tRNA Synthetase for Incorporation of L-3,4-Dihydroxyphenylalanine (L-DOPA).
Journal Angew Chem Int Ed Engl
Abstract The catechol group of 3,4-dihydroxyphenylalanine (L-DOPA) derived from L-tyrosine oxidation is a key post-translational modification (PTM) in many protein biomaterials and has potential as a bioorthogonal handle for precision protein conjugation applications such as antibody-drug conjugates. Despite this potential, indiscriminate enzymatic modification of exposed tyrosine residues or complete replacement of tyrosine using auxotrophic hosts remains the preferred method of introducing the catechol moiety into proteins, which precludes many protein engineering applications. We have developed new orthogonal translation machinery to site-specifically incorporate L-DOPA into recombinant proteins and a new fluorescent biosensor to selectively monitor L-DOPA incorporation in vivo. We show simultaneous biosynthesis and incorporation of L-DOPA and apply this translation machinery to engineer a novel metalloprotein containing a DOPA-Fe chromophore.
Volume 60(27)
Pages 14811-14816
Published 2021-6-25
DOI 10.1002/anie.202100579
PMID 33871147
PMC PMC8217333
MeSH Amino Acyl-tRNA Synthetases / chemistry Amino Acyl-tRNA Synthetases / metabolism* Dihydroxyphenylalanine / chemistry Dihydroxyphenylalanine / metabolism* Models, Molecular Molecular Structure
DNA material B95. delta A (RDB13711)