RRC ID 69172
Author Ishiguro KI, Matsuura K, Tani N, Takeda N, Usuki S, Yamane M, Sugimoto M, Fujimura S, Hosokawa M, Chuma S, Ko MSH, Araki K, Niwa H.
Title MEIOSIN Directs the Switch from Mitosis to Meiosis in Mammalian Germ Cells.
Journal Dev Cell
Abstract The mechanisms regulating meiotic initiation in mammals are enigmatic. It is known that retinoic acid (RA) signaling plays a pivotal role during meiotic initiation. STRA8, which is expressed in response to RA, is thought to be a key factor promoting meiotic initiation. However, the specific role of STRA8 in meiotic initiation has remained elusive. Here, we identified MEIOSIN as a germ-cell-specific factor that associates with STRA8. MEIOSIN, like STRA8, is expressed in response to RA and plays an essential role in meiotic initiation in both males and females. Functional analyses revealed that MEIOSIN acts as a transcription factor together with STRA8, and that both factors are critical for driving meiotic gene activation. Furthermore, temporally restricted expression of MEIOSIN leads to meiotic entry decision during spermatogenesis. The present study demonstrates that MEIOSIN, in collaboration with STRA8, plays a central role in regulating the mitosis to meiosis germ cell fate decision in mammals.
Volume 52(4)
Pages 429-445.e10
Published 2020-2-24
DOI 10.1016/j.devcel.2020.01.010
PII S1534-5807(20)30011-3
PMID 32032549
MeSH Adaptor Proteins, Signal Transducing / physiology* Animals Cell Cycle* Cell Differentiation Female Gene Expression Regulation* Germ Cells / cytology Germ Cells / physiology* Male Meiosis* Mice Mice, Inbred C57BL Mice, Knockout Mitosis* Signal Transduction Spermatogenesis Transcription Factors / physiology*
IF 10.092
DNA material MEIOSIN-N (RDB) MEIOSIN-C (RDB18713) STRA8-His (RDB18714) STRA8-3xFLAG-HA-P2A-GFP (RDB18715)