論文 - 詳細
| RRC ID | 69176 |
|---|---|
| 著者 | Yasuhira S, Yasui A. |
| タイトル | Visible light-inducible photolyase gene from the goldfish Carassius auratus. |
| ジャーナル | J Biol Chem |
| Abstract |
By introducing and expressing a cDNA library constructed from mRNA of the cultured goldfish Carassius auratus cells in Escherichia coli, a gene encoding photolyase of the vertebrate was isolated, the first example from metazoa. The amino acid sequence deduced from the nucleotide sequence differs significantly from those of microorganisms. Five out of 6 tryptophan residues strictly conserved in photolyases from microorganisms and thought to play important roles in DNA and chromophore binding of the enzyme are substituted by other residues of different characteristics. By Northern analysis the expression of the photolyase gene was found to be induced more than 10 times by exposure of the cells to visible light. These results indicate a unique evolution of the photolyase gene and a novel mechanism of gene regulation, in which visible light triggers the production of the light-dependent enzyme for repair of DNA damages induced by harmful ultraviolet part of sunlight. |
| 巻・号 | 267(36) |
| ページ | 25644-7 |
| 公開日 | 1992-12-25 |
| PII | S0021-9258(18)35652-7 |
| PMID | 1339447 |
| MeSH | Amino Acid Sequence Animals Bacteria / enzymology Bacteria / genetics Base Sequence Cloning, Molecular DNA / genetics DNA / isolation & purification Deoxyribodipyrimidine Photo-Lyase / biosynthesis Deoxyribodipyrimidine Photo-Lyase / genetics* Enzyme Induction Escherichia coli / genetics Escherichia coli / radiation effects* Fungi / enzymology Fungi / genetics Gene Expression Regulation, Enzymologic / radiation effects* Gene Library Genes / radiation effects* Goldfish Kinetics Light Molecular Sequence Data Recombinant Proteins / biosynthesis Sequence Homology, Amino Acid |
| IF | 4.238 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME9936 |