論文 - 詳細
| RRC ID | 69188 |
|---|---|
| 著者 | Sakai S, Liu Y, Mah EJ, Taya M. |
| タイトル | Horseradish peroxidase/catalase-mediated cell-laden alginate-based hydrogel tube production in two-phase coaxial flow of aqueous solutions for filament-like tissues fabrication. |
| ジャーナル | Biofabrication |
| Abstract |
We report a method for preparing cell-laden hydrogel tubes. This method uses a coaxial double-orifice spinneret, simpler than triple-orifice spinnerets which have been used for preparing similar constructs. The intended application was to create a template for preparing filament-like structures composed of two heterogeneous living cell layers. An aqueous solution containing an alginate derivative possessing phenolic hydroxyl moieties (Alg-Ph), catalase and horseradish peroxidase (HRP) was extruded into an ambient flow of H(2)O(2) aqueous solution. This operation enabled the Alg-Ph solution to be gellable through a HRP-catalyzed reaction, cross-linking the Ph moieties together. By altering flow rates of the Alg-Ph and H(2)O(2) solutions along with the concentrations of catalase and H(2)O(2), the diameter and membrane thickness of the hydrogel tubes were controllable between 250-550 µm and 70-140 µm, respectively. The viability of the HeLa cells enclosed in the hydrogel tubes with a diameter of 300 µm and a membrane thickness of 80 µm was 95.4%. Subsequently, the enclosed HeLa cells grew and filled the hollow core. A filament-like structure of HeLa cells covered with a layer of fibroblast 10T1/2 cells was obtained when confluency of fibroblast 10T1/2 cells was reached and the hydrogel matrix was degraded with alginate lyase. |
| 巻・号 | 5(1) |
| ページ | 015012 |
| 公開日 | 2013-3-1 |
| DOI | 10.1088/1758-5082/5/1/015012 |
| PMID | 23319520 |
| MeSH | Alginates / chemistry* Biocompatible Materials / chemical synthesis* Biocompatible Materials / chemistry Bioengineering / instrumentation Bioengineering / methods* Catalase / chemistry Cell Proliferation Cell Survival Fibroblasts / cytology Glucuronic Acid / chemistry HeLa Cells Hexuronic Acids / chemistry Horseradish Peroxidase / chemistry Humans Hydrogel, Polyethylene Glycol Dimethacrylate / chemical synthesis* Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry Tissue Engineering / instrumentation* Tissue Scaffolds / chemistry* |
| IF | 8.213 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa 10T1/2(RCB0247) |