論文 - 詳細
| RRC ID | 69198 |
|---|---|
| 著者 | Zhang L, Mao B, Zhao X, Yuan Y, Wang W, Lin S. |
| タイトル | Translation regulatory long non-coding RNA 1 (TRERNA1) sponges microRNA-23a to suppress granulosa cell apoptosis in premature ovarian failure. |
| ジャーナル | Bioengineered |
| Abstract |
Translation regulatory long non-coding RNA 1 (TRERNA1) plays critical roles in cancer biology. We predicted the direct interaction of TRERNA1 with microRNA (miR)-23a, which promotes granulosa apoptosis. Granulosa apoptosis is involved in premature ovarian failure (POF). This study was therefore carried out to explore the involvement of TRERNA1 and miR-23a in POF. The expression of TRERNA1 and miR-23a in POF and control groups were detected by RT-qPCRs. The subcellular locations of TRERNA1 in granulosa cell line COV434 was detected by subcellular fractionation assay. The interaction between TRERNA1 and miR-23a was predicted using IntaRNA2.0. The direct interaction between COV434 and miR-23a was explored with RNA pull-down assay. In granulosa cells, the direct interaction between TRERNA1 and miR-23a was verified by overexpression assay. Cell apoptosis assay was performed to evaluate cell apoptosis. Both TRERNA1 and miR-23a were downregulated in POF. In addition, TRERNA1 was detected in both cytoplasm and nuclear samples of granulosa cells, and directly interacted with miR-23a. TRERNA1 did not affect the expression of miR-23a in granulosa cells, while TRERNA1 suppressed the role of miR-23a in enhancing cell apoptosis. In conclusion, TRERNA1 may sponge miR-23a to suppress granulosa cell apoptosis in POF. |
| 巻・号 | 13(2) |
| ページ | 2173-2180 |
| 公開日 | 2022-2-1 |
| DOI | 10.1080/21655979.2021.2023802 |
| PMID | 35034562 |
| PMC | PMC8973726 |
| MeSH | Adult Apoptosis* Cell Line, Tumor Female Granulosa Cells / metabolism* Granulosa Cells / pathology Humans MicroRNAs / genetics MicroRNAs / metabolism* Primary Ovarian Insufficiency / genetics Primary Ovarian Insufficiency / metabolism* Primary Ovarian Insufficiency / pathology RNA, Long Noncoding / genetics RNA, Long Noncoding / metabolism* |
| IF | 1.639 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | KGN(RCB1154) |