| RRC ID |
69198
|
| 著者 |
Zhang L, Mao B, Zhao X, Yuan Y, Wang W, Lin S.
|
| タイトル |
Translation regulatory long non-coding RNA 1 (TRERNA1) sponges microRNA-23a to suppress granulosa cell apoptosis in premature ovarian failure.
|
| ジャーナル |
Bioengineered
|
| Abstract |
Translation regulatory long non-coding RNA 1 (TRERNA1) plays critical roles in cancer biology. We predicted the direct interaction of TRERNA1 with microRNA (miR)-23a, which promotes granulosa apoptosis. Granulosa apoptosis is involved in premature ovarian failure (POF). This study was therefore carried out to explore the involvement of TRERNA1 and miR-23a in POF. The expression of TRERNA1 and miR-23a in POF and control groups were detected by RT-qPCRs. The subcellular locations of TRERNA1 in granulosa cell line COV434 was detected by subcellular fractionation assay. The interaction between TRERNA1 and miR-23a was predicted using IntaRNA2.0. The direct interaction between COV434 and miR-23a was explored with RNA pull-down assay. In granulosa cells, the direct interaction between TRERNA1 and miR-23a was verified by overexpression assay. Cell apoptosis assay was performed to evaluate cell apoptosis. Both TRERNA1 and miR-23a were downregulated in POF. In addition, TRERNA1 was detected in both cytoplasm and nuclear samples of granulosa cells, and directly interacted with miR-23a. TRERNA1 did not affect the expression of miR-23a in granulosa cells, while TRERNA1 suppressed the role of miR-23a in enhancing cell apoptosis. In conclusion, TRERNA1 may sponge miR-23a to suppress granulosa cell apoptosis in POF.
|
| 巻・号 |
13(2)
|
| ページ |
2173-2180
|
| 公開日 |
2022-2-1
|
| DOI |
10.1080/21655979.2021.2023802
|
| PMID |
35034562
|
| PMC |
PMC8973726
|
| MeSH |
Adult
Apoptosis*
Cell Line, Tumor
Female
Granulosa Cells / metabolism*
Granulosa Cells / pathology
Humans
MicroRNAs / genetics
MicroRNAs / metabolism*
Primary Ovarian Insufficiency / genetics
Primary Ovarian Insufficiency / metabolism*
Primary Ovarian Insufficiency / pathology
RNA, Long Noncoding / genetics
RNA, Long Noncoding / metabolism*
|
| IF |
1.639
|
| リソース情報 |
| ヒト・動物細胞 |
KGN(RCB1154) |
