| RRC ID |
69213
|
| 著者 |
Nishimoto T, Kashiwagi K, Saito N, Shirai Y.
|
| タイトル |
Both C1B domain and pseudosubstrate region are necessary for saturated fatty acid-induced translocation of εPKC to the plasma membrane: distinct role of intramolecular domains for different translocation.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
It is well known that protein kinase C (PKC) shows different translocation depending on subtype and stimulation, contributing to the physiological importance of the enzyme. However, molecular mechanism causing the different translocation has been unknown. Therefore, using GFP-tagged mutant εPKC, we attempted to identify the intramolecular domains required for saturated fatty acid-induced translocation of εPKC to the plasma membrane, and compared with those necessary for unsaturated fatty acid-induced translocation to the Golgi complex. We found that, unlike in the case of unsaturated fatty-acid induced translocation, both C1B domain and pseudosubstrate region are necessary for the saturated fatty acid-induced translocation of εPKC to the plasma membrane. The results suggest that different domains of PKC mediate distinct translocation depending on different stimulations, contributing to their subtype- and stimulation-specific functions.
|
| 巻・号 |
432(2)
|
| ページ |
384-8
|
| 公開日 |
2013-3-8
|
| DOI |
10.1016/j.bbrc.2013.01.048
|
| PII |
S0006-291X(13)00116-2
|
| PMID |
23353652
|
| MeSH |
Animals
COS Cells
Cell Membrane / enzymology*
Chlorocebus aethiops
Fatty Acids / metabolism*
Fatty Acids / pharmacology
HEK293 Cells
Humans
Protein Kinase C / chemistry
Protein Kinase C / genetics
Protein Kinase C / metabolism*
Protein Structure, Tertiary / genetics
Protein Transport
Rats
|
| IF |
2.985
|
| リソース情報 |
| ヒト・動物細胞 |
COS-7(RCB0539)
293(RCB1637) |
