RRC ID 69217
著者 Kanno T, Tsuchiya A, Tanaka A, Nishizaki T.
タイトル The linoleic acid derivative DCP-LA increases membrane surface localization of the α7 ACh receptor in a protein 4.1N-dependent manner.
ジャーナル Biochem J
Abstract In yeast two-hybrid screening, protein 4.1N, a scaffolding protein, was identified as a binding partner of the α7 ACh (acetylcholine) receptor. For rat hippocampal slices, the linoleic acid derivative DCP-LA {8-[2-(2-pentyl-cyclopropylmethyl)-cyclopropyl]-octanoic acid} increased the association of the α7 ACh receptor with 4.1N, and the effect was inhibited by GF109203X, an inhibitor of PKC (protein kinase C), although DCP-LA did not induce PKC phosphorylation of 4.1N. For PC-12 cells, the presence of the α7 ACh receptor in the plasma membrane fraction was significantly suppressed by knocking down 4.1N. DCP-LA increased the presence of the α7 ACh receptor in the plasma membrane fraction, and the effect was still inhibited by knocking down 4.1N. In the monitoring of α7 ACh receptor mobilization, DCP-LA enhanced signal intensities for the α7 ACh receptor at the membrane surface in PC-12 cells, which was clearly prevented by knocking down 4.1N. Taken together, the results of the present study show that 4.1N interacts with the α7 ACh receptor and participates in the receptor tethering to the plasma membrane. The results also indicate that DCP-LA increases membrane surface localization of the α7 ACh receptor in a 4.1N-dependent manner under the control of PKC, but without phosphorylating 4.1N.
巻・号 450(2)
ページ 303-9
公開日 2013-3-1
DOI 10.1042/BJ20121348
PII BJ20121348
PMID 23256752
MeSH Animals Caprylates / pharmacology* Cell Membrane / metabolism* HEK293 Cells Hippocampus / drug effects Hippocampus / physiology Humans Linoleic Acids / pharmacology* Male Patch-Clamp Techniques Rats Rats, Wistar Receptors, Nicotinic / analysis* Receptors, Nicotinic / metabolism* Transfection alpha7 Nicotinic Acetylcholine Receptor
IF 4.097
リソース情報
ヒト・動物細胞 PC-12(RCB0009) 293(RCB1637)