| RRC ID |
69242
|
| 著者 |
Kokuryo D, Anraku Y, Kishimura A, Tanaka S, Kano MR, Kershaw J, Nishiyama N, Saga T, Aoki I, Kataoka K.
|
| タイトル |
SPIO-PICsome: development of a highly sensitive and stealth-capable MRI nano-agent for tumor detection using SPIO-loaded unilamellar polyion complex vesicles (PICsomes).
|
| ジャーナル |
J Control Release
|
| Abstract |
Size controllable polyion complex vesicles (PICsomes), composed of biocompatible poly(ethylene glycol) (PEG) and poly(amino acid)s, have an extremely prolonged lifetime in the bloodstream that enables them to accumulate effectively in tumors via the enhanced permeability and retention (EPR) effect. The purpose of this study was to use PICsomes to synthesize a highly sensitive MRI contrast agent for more precise tumor detection. We synthesized SPIO-Cy5-PICsomes (superparamagnetic iron oxide nanoparticle-loaded Cy5-cross-linked Nano-PICsomes) and characterized them using dynamic light scattering and transmission electron microscopy in vitro and evaluated their ability to detect subcutaneously grafted tumors in vivo with MRI. The transverse relaxivity (r2) of the SPIO-Cy5-PICsomes (r2=663±28mM(-1)s(-1)) was 2.54 times higher than that of bare clinically-used SPIO. In in vivo MRI experiments on mice subcutaneously grafted with colon-26 tumor cells, the tumor signal was significantly altered at 3h after SPIO-Cy5-PICsome administration and persisted for at least 24h. Small and early-stage in vivo tumors (3days after grafting, approximately 4mm(3)) were also clearly detected with MRI. SPIO-loaded PICsomes are sensitive MRI contrast agents that can act as a powerful nanocarrier to detect small tumors for early diagnosis.
|
| 巻・号 |
169(3)
|
| ページ |
220-7
|
| 公開日 |
2013-8-10
|
| DOI |
10.1016/j.jconrel.2013.03.016
|
| PII |
S0168-3659(13)00159-4
|
| PMID |
23542239
|
| MeSH |
Amino Acids / chemical synthesis
Amino Acids / chemistry
Animals
Carbocyanines / chemical synthesis
Carbocyanines / chemistry
Contrast Media / chemical synthesis
Contrast Media / chemistry*
Female
Magnetic Resonance Imaging / methods
Magnetite Nanoparticles / chemistry*
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasms / diagnosis*
Polyethylene Glycols / chemical synthesis
Polyethylene Glycols / chemistry
|
| IF |
7.727
|
| リソース情報 |
| ヒト・動物細胞 |
Colon-26(RCB2657) |
