論文 - 詳細
| RRC ID | 69285 |
|---|---|
| 著者 | Kato Y, Hagiwara M, Ishihara Y, Isoda R, Sugiura S, Komatsu T, Ishida N, Noguchi T, Matsushita K. |
| タイトル | TNF-α augmented Porphyromonas gingivalis invasion in human gingival epithelial cells through Rab5 and ICAM-1. |
| ジャーナル | BMC Microbiol |
| Abstract |
BACKGROUND:Tumor necrosis factor alpha (TNF-α) plays a central role in the initiation and maintenance of immune responses to periodontopathic bacteria. However, excess TNF-α leads to dysregulated immune responses and progression of periodontitis. Porphyromonas gingivalis (P. gingivalis) invades gingival epithelial cells and then multiplies and survives for a long period. Additionally, increment of TNF-α in periodontal sites is associated with a high prevalence of gram-negative anaerobes such as P. gingivalis. However, it has not been determined whether TNF-α affects invasion of P. gingivalis in periodontal tissues. RESULTS:We examined the effect of TNF-α on invasion of P. gingivalis in gingival epithelial cells and clarified the mechanism by which TNF-α augments invasion of P. gingivalis. Invasion of P. gingivalis into Ca9-22 cells was augmented by stimulation with TNF-α and it was inhibited by treatment with an antibody to TNF receptor-1. TNF-α increased production of ICAM-1, and P. gingivalis invasion was inhibited by an antibody to ICAM-1 in Ca9-22 cells. Silencing of Rab5 mRNA inhibited P. gingivalis invasion. Furthermore, the JNK inhibitor SP600125 inhibited invasion of P. gingivalis and also decreased the active form of Rab5 in Ca9-22 cells. CONCLUSION:TNF-α augments invasion of P. gingivalis in human gingival epithelial cells through increment of ICAM-1 and activation of Rab5. These phenomena may contribute to persistent infection of P. ginigvalis and prolongation of immune responses in periodontal tissues. |
| 巻・号 | 14 |
| ページ | 229 |
| 公開日 | 2014-9-3 |
| DOI | 10.1186/s12866-014-0229-z |
| PII | s12866-014-0229-z |
| PMID | 25179218 |
| PMC | PMC4159534 |
| MeSH | Cell Line Endocytosis* Epithelial Cells / drug effects* Epithelial Cells / microbiology* Humans Intercellular Adhesion Molecule-1 / metabolism* Porphyromonas gingivalis / growth & development Porphyromonas gingivalis / physiology* Tumor Necrosis Factor-alpha / immunology Tumor Necrosis Factor-alpha / metabolism* rab5 GTP-Binding Proteins / metabolism* |
| IF | 2.989 |
| リソース情報 | |
| ヒト・動物細胞 | Ca9-22(RCB1976) |