RRC ID 69354
Author Suzuki S, Sasaki K, Fukazawa T, Kubo T.
Title Xenopus laevis il11ra.L is an experimentally proven interleukin-11 receptor component that is required for tadpole tail regeneration.
Journal Sci Rep
Abstract Xenopus laevis tadpoles possess high regenerative ability and can regenerate functional tails after amputation. An early event in regeneration is the induction of undifferentiated cells that form the regenerated tail. We previously reported that interleukin-11 (il11) is upregulated immediately after tail amputation to induce undifferentiated cells of different cell lineages, indicating a key role of il11 in initiating tail regeneration. As Il11 is a secretory factor, Il11 receptor-expressing cells are thought to mediate its function. X. laevis has a gene annotated as interleukin 11 receptor subunit alpha on chromosome 1L (il11ra.L), a putative subunit of the Il11 receptor complex, but its function has not been investigated. Here, we show that nuclear localization of phosphorylated Stat3 induced by Il11 is abolished in il11ra.L knocked-out culture cells, strongly suggesting that il11ra.L encodes an Il11 receptor component. Moreover, knockdown of il11ra.L impaired tadpole tail regeneration, suggesting its indispensable role in tail regeneration. We also provide a model showing that Il11 functions via il11ra.L-expressing cells in a non-cell autonomous manner. These results highlight the importance of il11ra.L-expressing cells in tail regeneration.
Volume 12(1)
Pages 1903
Published 2022-2-3
DOI 10.1038/s41598-022-05954-w
PII 10.1038/s41598-022-05954-w
PMID 35115663
PMC PMC8814168
MeSH Animals Cell Line Cell Proliferation* / drug effects Gene Expression Regulation, Developmental Interleukin-11 / pharmacology Interleukin-11 Receptor alpha Subunit / agonists Interleukin-11 Receptor alpha Subunit / genetics Interleukin-11 Receptor alpha Subunit / metabolism* Larva / drug effects Larva / genetics Larva / growth & development Larva / metabolism* Phosphorylation Regeneration* / drug effects STAT3 Transcription Factor / metabolism Signal Transduction Tail / drug effects Tail / embryology Tail / metabolism* Xenopus Proteins / genetics Xenopus Proteins / metabolism* Xenopus laevis / embryology Xenopus laevis / genetics Xenopus laevis / metabolism*
IF 3.998
Human and Animal Cells XTC-YF(RCB0771)