RRC ID 69368
著者 Yamamoto K, Miura H, Ishida M, Mii Y, Kinoshita N, Takada S, Ueno N, Sawai S, Kondo Y, Aoki K.
タイトル Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis.
ジャーナル Nat Commun
Abstract Actomyosin contractility generated cooperatively by nonmuscle myosin II and actin filaments plays essential roles in a wide range of biological processes, such as cell motility, cytokinesis, and tissue morphogenesis. However, subcellular dynamics of actomyosin contractility underlying such processes remains elusive. Here, we demonstrate an optogenetic method to induce relaxation of actomyosin contractility at the subcellular level. The system, named OptoMYPT, combines a protein phosphatase 1c (PP1c)-binding domain of MYPT1 with an optogenetic dimerizer, so that it allows light-dependent recruitment of endogenous PP1c to the plasma membrane. Blue-light illumination is sufficient to induce dephosphorylation of myosin regulatory light chains and a decrease in actomyosin contractile force in mammalian cells and Xenopus embryos. The OptoMYPT system is further employed to understand the mechanics of actomyosin-based cortical tension and contractile ring tension during cytokinesis. We find that the relaxation of cortical tension at both poles by OptoMYPT accelerated the furrow ingression rate, revealing that the cortical tension substantially antagonizes constriction of the cleavage furrow. Based on these results, the OptoMYPT system provides opportunities to understand cellular and tissue mechanics.
巻・号 12(1)
ページ 7145
公開日 2021-12-8
DOI 10.1038/s41467-021-27458-3
PII 10.1038/s41467-021-27458-3
PMID 34880255
PMC PMC8654997
MeSH Actin Cytoskeleton / metabolism Actomyosin / metabolism* Animals Cell Membrane / metabolism Cell Movement Cytokinesis / genetics Cytokinesis / physiology* Cytoskeletal Proteins / metabolism Dogs Female Intercellular Junctions Madin Darby Canine Kidney Cells Male Mechanical Phenomena Morphogenesis Muscle Contraction Myosin Type II / metabolism Myosin-Light-Chain Phosphatase / metabolism Optogenetics* Synthetic Biology Xenopus
IF 12.121
リソース情報
ヒト・動物細胞 MDCK(RCB0995)